Kit ɗin bincike don Estradiol(Fluorescence immunochromatographic assay)
Don bincikar in vitro amfani kawai
Da fatan za a karanta wannan fakitin a hankali kafin amfani kuma a bi umarnin sosai.Ba za a iya tabbatar da amincin sakamakon kima ba idan akwai wasu sabani daga umarnin a cikin wannan fakitin.

AMFANI DA NUFIN
Kit ɗin bincike don Estradiol (ƙaddamar da fluorescence immunochromatographic assay) shine gwajin immunochromatographic mai haske don gano ƙimar Estradiol (E2) a cikin jini ko jini na ɗan adam, wanda galibi ana amfani dashi don kimanta matakan Estradiol. dole ne a tabbatar da wasu hanyoyin.Anyi nufin wannan gwajin don amfanin ƙwararrun kiwon lafiya kawai.

TAKAITACCEN
Estradiol (E2) shine hormone mafi mahimmanci kuma mafi aiki a cikin estrogen. Nauyin kwayoyinsa shine 272.3 D. Gabaɗaya, ga mata marasa ciki, E2 galibi ana ɓoye su ta hanyar kwasfa da ƙwayoyin granular da ƙwayoyin luteal yayin haɓakar follicular. E2 galibi ana fitar da shi ne ta wurin mahaifa, yayin da maza ke samar da su ta hanyar ƙwararru. Bayan E2 ya shiga cikin jini, 1% zuwa 3% ba sa ɗaure ga sunadaran, 40% suna ɗaure ga hormone binding globulin (SHBG), da sauransu. albumin, metabolize daga hanta zuwa ruwa mai narkewa sulfates ko gluconaldehyde esters, da kuma fitar da fitsari.E2 ne mai muhimmanci hormone nuna alama ga kimanta aikin ovarian.

KA'IDAR HANYA
An lulluɓe membrane na na'urar gwajin tare da haɗin BSA da Estradiol akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa.Alamar alamar an lullube ta da alamar fluorescence ta anti E2 antibody da zomo IgG a gaba.Lokacin gwada samfurin, E2 a cikin samfurin haɗa tare da mai walƙiya alama anti E2 antibody, da samar da cakuda rigakafi.A karkashin aikin immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun ya wuce gwajin yankin, The free kyalli alama za a hade tare da Estradiol a kan membrane.The taro na Estradiol ne korau daidaituwa ga haske sigina, da kuma Ana iya gano maida hankali na Estradiol a cikin samfurin ta hanyar gwajin immunoassay fluorescence.

REAgents DA KAYAN DA AKA BAYAR

Abubuwan kunshin 25T:
.Katin gwajin daidaiku da foil ɗin da aka shayar da shi tare da 25T na busa
.Mafita 25T
.B Magani 1
. Kunshin saka 1

KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
Samfurin tarin akwati, mai ƙidayar lokaci

MISALI TATTAUNAWA DA AJIYA
1. Samfuran da aka gwada na iya zama jini, heparin anticoagulant plasma ko EDTA anticoagulant plasma.
2.According ga daidaitattun dabarun tattara samfurin.Za'a iya adana ruwan magani ko samfurin plasma a cikin firiji a 2-8 ℃ na tsawon kwanaki 7 kuma ana kiyaye cryopreservation ƙasa -15 ° C na watanni 6.
.Duk samfurin guje wa daskare-narke hawan keke.

HANYAR ASSAY
Hanyar gwaji na kayan aiki duba jagorar immunoanalyzer.Hanyar gwajin reagent shine kamar haka
1.Lay a gefe duk reagents da samfurori zuwa dakin zafin jiki.
2.Bude Portable Immune Analyzer (WIZ-A101), shigar da kalmar sirri shiga asusu bisa ga tsarin aiki na kayan aiki, da kuma shigar da ganowa dubawa.
3.Scan da lambar haƙori don tabbatar da gwajin abu.
3.Dauki katin gwaji daga jakar foil.
4.Saka katin gwaji a cikin ramin katin, duba lambar QR, kuma ƙayyade abin gwajin.
5.Add 30μL serum ko plasma samfurin zuwa A bayani, da kuma Mix da kyau.
6.Add 20μL B bayani zuwa ga cakuda na sama, da kuma Mix da kyau.
Bar cakuda don20mintuna.
Ƙara cakuda 80μL zuwa samfurin rijiyar katin.
Danna maɓallin "misali gwajin", bayan minti 10, kayan aiki za su gano katin gwajin ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aiki, kuma ya yi rikodin / buga sakamakon gwajin.
Koma zuwa umarnin Mai Binciken Immune Mai ɗaukar nauyi (WIZ-A101).

SAKAMAKON JARRABAWA DA FASSARA

.Bayanan da ke sama shine tazara tazara da aka kafa don gano bayanan wannan kit ɗin, kuma ana ba da shawarar cewa kowane ɗakin gwaje-gwaje ya kamata ya kafa tazara tazara don mahimmancin asibiti da ya dace na yawan jama'a a wannan yanki.
.Ƙaƙƙarwar estradiol ya fi girma fiye da ma'anar tunani, kuma ya kamata a cire sauye-sauye na ilimin lissafi ko amsawar damuwa.Hakika maras kyau, ya kamata ya hada da ganewar asali na asibiti.
.Sakamakon wannan hanyar yana aiki ne kawai ga kewayon tunani da aka kafa ta wannan hanyar, kuma sakamakon ba ya daidaita kai tsaye da sauran hanyoyin.
.Wasu dalilai kuma na iya haifar da kurakurai a cikin sakamakon ganowa, gami da dalilai na fasaha, kurakuran aiki da sauran abubuwan samfuri.

AJIYA DA KWANTA
1.The kit ne 18 watanni shiryayye-rai daga ranar da aka yi.Ajiye kayan da ba a yi amfani da su ba a 2-30 ° C.KAR KA DANKE.Kar a yi amfani da bayan ranar karewa.
2.Kada ku buɗe jakar da aka rufe har sai kun shirya don yin gwaji, kuma ana ba da shawarar yin amfani da gwajin amfani ɗaya a ƙarƙashin yanayin da ake buƙata (zazzabi 2-35 ℃, zafi 40-90%) a cikin 60 mins da sauri. kamar yadda zai yiwu.
3.Sample diluent ana amfani da shi nan da nan bayan an bude shi.

GARGADI DA TSIRA
.Kit ɗin yakamata a rufe shi kuma a kiyaye shi daga danshi.
.Duk samfurori masu inganci za a inganta su ta wasu hanyoyin.
.Duk samfuran za a kula da su azaman masu gurɓata yanayi.
.KADA KA yi amfani da reagent da ya ƙare.
.KADA KA musanya reagents tsakanin kits tare da kuri'a daban-daban.
.KADA KA sake amfani da katunan gwaji da duk wani kayan haɗi da za'a iya zubarwa.
.Misoperation, wuce kima ko kadan samfurin iya haifar da sabawa sakamakon.

LIMITATION
Kamar yadda yake tare da kowane gwajin amfani da ƙwayoyin rigakafi na linzamin kwamfuta, akwai yuwuwar kutsawa daga ƙwayoyin rigakafin linzamin kwamfuta na ɗan adam (HAMA) a cikin samfurin.Samfura daga marasa lafiya waɗanda suka karɓi shirye-shiryen rigakafin ƙwayoyin cuta na monoclonal don ganewar asali ko jiyya na iya ƙunsar HAMA.Irin waɗannan samfurori na iya haifar da sakamako mara kyau na ƙarya ko ƙarya.
.Wannan sakamakon gwajin shine kawai don tunani na asibiti, bai kamata ya zama tushen kawai don ganewar asibiti da magani ba, kulawar likitocin marasa lafiya ya kamata a yi la'akari sosai tare da alamunta,
. tarihin likitanci, sauran gwaje-gwaje na dakin gwaje-gwaje, amsa magani, cututtukan cututtuka da sauran bayanai.
.Wannan reagent ana amfani dashi kawai don gwajin jini da jini.Maiyuwa baya samun ingantaccen sakamako idan aka yi amfani da shi don wasu samfura kamar yau da fitsari da sauransu.
HALAYEN YI

REFERENCES
1.Hansen JH, et al.HAMA Tsangwama tare da Murine Monoclonal Antibody-Based Immunoassays[J].J na Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Yanayin Heterophilic Antibodies da Matsayin Tsangwama na Immunoassay[J].J na Clin Immunoassay,1992,15:108-114.

Maɓalli ga alamomin da aka yi amfani da su:

	In Vitro Diagnostic Medical Na'urar
	Mai ƙira
	Adana a 2-30 ℃
	Ranar Karewa
	Kada a sake amfani
	HANKALI
	Tuntuɓi Umarnin Don Amfani

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