Kete Tātaritanga（Koura Kororā）mō Calprotectin
Mō te whakamahinga tātaritanga in vitro anake
Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA
Ko te Kete Tātaritanga mō te Calprotectin(cal) he whakamātautau immunochromatographic koura colloidal mō te whakatau haurua-tau o te cal mai i ngā paru tangata, he mea nui te uara tātaritanga tāpiri mō te mate puku mumura. He matū tirotiro tēnei whakamātautau. Me whakaū katoa ngā tauira pai mā ētahi atu tikanga. Ko te tikanga o tēnei whakamātautau he mea whakamahi noa mā ngā tohunga hauora. I tēnei wā, ka whakamahia tēnei whakamātautau mō te IVD, kāore e hiahiatia he taputapu tāpiri.

WHAKARĀPOPOTONGA
He heterodimer a Cal, he mea tito mai i te MRP 8 me te MRP 14. Kei roto i te cytoplasm o ngā neutrophils, ā, kei runga i ngā kiriuhi pūtau mononuclear. He pūmua wāhanga whakapeka a Cal, he wāhanga pumau mō te wiki kotahi i roto i te paru tangata, ā, kua whakatauhia hei tohu mate puku mumura. He whakamātautau ā-tirotiro ngāwari te kete, he whakamātautau ā-whakamātautau ā-kanohi e kitea ai te cal i roto i te paru tangata, he tino aro ki te kite me te tino motuhake. Ko te whakamātautau e hangai ana ki te kaupapa tauhohenga paturopi takirua tino motuhake me ngā tikanga tātari whakamātautau immunochromatographic koura, ka taea te whakaputa hua i roto i te 15 meneti.

TE MĀTĀPONO O TE TIKANGA
Kei te rīpene he paninga anti-cal McAb i te rohe whakamātautau me te paturopi anti-rāpeti koati IgG i te rohe whakahaere, e herea ana ki te chromatography membrane i mua. Ka pania te papa tapanga ki te koura colloidal kua tohua he anti-cal McAb me te paturopi rapeti kua tohua he koura colloidal IgG i mua. I te whakamātautau i te tauira pai, ka honoa te cal i roto i te tauira ki te koura colloidal kua tohua he anti-cal McAb, ka hanga he matatini ārai mate, i te mea ka tukuna kia heke i te rīpene whakamātautau, ka hopukina te matatini honohono cal e te paninga anti-cal McAb i runga i te membrane ka hanga he matatini "paninga anti-cal McAb-cal-colloidal koura kua tohua he anti-cal McAb", ka puta he roopu whakamātautau tae i te rohe whakamātautau. He hononga pai te kaha o te tae ki te ihirangi cal. Kāore he roopu whakamātautau e puta mai i te tauira kino nā te korenga o te matatini honohono koura colloidal. Ahakoa kei roto te cal i te tauira, kāore rānei, ka puta he raina whero i te rohe tohutoro me te rohe whakahaere kounga, e kiia ana ko ngā paerewa kounga ā-roto o te umanga.

NGĀ RĀWENI ME NGĀ RAUEMI I TUKUNA
Ngā wāhanga mōkihi 25T：
Kāri whakamātautau takitahi he pepa konutea kua kapi ki te whakamaroke
.Tauira wairewa: ko ngā kai he 20mM pH7.4PBS
.Dispette
.Whakaurunga mōkihi

NGĀ RAUEMI E HIAHIATIA ANA ENGARI KĀORE I HOATU

Ipu kohinga tauira, taima

TE KOHINGA TAUIRA ME TE ROKIROKI
Whakamahia he ipu ma, ka taea te whakamahi hei kohi i ngā tauira paru hou, ka whakamātauria tonutia. Ki te kore e taea te whakamātau tonutia, rongoatia ki te 2-8°C mō ngā hāora 12, ki raro rānei i te -15°C mō ngā marama e 4.

TE TIKANGA WHAKAMĀTAUTAU
1. Tangohia te rākau tauira kua whakaurua ki roto i te tauira paru, kātahi ka whakahokia te rākau tauira, ka whakapiri kia mau, ka rūrū kia pai, ka tuaruatia te mahi kia toru ngā wā. Mā te whakamahi rānei i te rākau tauira kua tohua kia 50mg te tauira paru, ka whakauru ki roto i te ngongo tauira paru kei roto te waimeha tauira, ka whakapiri kia mau.
2. Whakamahia he pipette tango tauira, tangohia te tauira paru angiangi mai i te tūroro mate korere, kātahi ka tāpirihia kia 3 ngā pata (tata ki te 100uL) ki te ngongo tauira paru, ka rūrū pai, ka waiho ki tahaki.
3. Tangohia te kāri whakamātautau mai i te putea pepa konutea, whakatakotoria ki runga i te tēpu papatahi, ka tohua.
4. Tangohia te taupoki i te ngongo tauira ka maka atu ngā pata tuatahi e rua o te tauira kua whakaranua, tāpirihia kia 3 ngā pata (tata ki te 100µL) o te tauira kua whakaranua kia poutū, ka ata ringihia ki roto i te poka tauira o te kāri me te putanga kua whakaratohia, tīmatahia te wā.
5. Me pānui te hua i roto i ngā meneti 10-15, ā, kāore e whai mana i muri i ngā meneti 15.

NGĀ HUA WHAKAMĀTAUTAU ME TE WHAKAMĀRAMATANGA

TE ROKIROKI ME TE PŪMAU
E 24 marama te roa o te oranga o te kete mai i te rā i hangaia ai. Penapenahia ngā kete kāore anō kia whakamahia ki te 2-30°C. Kaua e whakatuwhera i te putea hiri kia rite rā anō koe ki te mahi i tētahi whakamātautau.
NGĀ WHAKATŪPATO ME NGĀ WHAKATŪPATO
1. Me hiri te kete, ā, me tiaki hoki i te makuku¹.
2. Kaua e whakamahia te tauira kua roa rawa te whakanoho, kua whakatiohia rānei, kua whakarewahia anō hei whakamātautau
3. Ki te nui rawa ngā tauira paru, ki te matotoru rānei, ka kino pea ngā tauira kua whakakōkītia ki te kāri whakamātautau, tēnā whakamahia te pūwero hei whakamātau i te tauira kua whakakōkītia.
4. Mā te hē o te mahi, te nui rawa, te iti rānei o te tauira ka rerekē ngā hua.

WHAKAWHĀITI
1. Hei tohutoro haumanu anake tēnei hua whakamātautau, kaua e waiho hei pūtake anake mō te tātaritanga haumanu me te maimoatanga, me whai whakaaro whānui te whakahaere haumanu o ngā tūroro me ōna tohu, hītori hauora, whakamātautau taiwhanga kē atu, urupare maimoatanga, mate urutā me ētahi atu mōhiohio.².
2. Ka whakamahia anake tēnei matū hei whakamātautau paru. Tērā pea kāore e tika te hua ina whakamahia mō ētahi atu tauira pēnei i te huware me te mimi me ētahi atu.