TE MATUA

Kua pania te kiriuhi o te taputapu whakamātautau ki te hononga o te BSA me te 25-(OH)VD i te rohe whakamātautau me te paturopi IgG ārai rāpeti koati i te rohe whakahaere. Kua pania ngā papa tohu ki te tohu fluorescence ārai rāpeti 25-(OH)VD me te IgG rāpeti i mua. I te whakamātautau i te tauira, ka honoa te 25-(OH)VD i roto i te tauira ki te paturopi ārai rāpeti kua tohua ki te fluorescence, ā, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti, ina paahitia e te matatini te rohe whakamātautau, ka honoa te tohu fluorescence kore utu ki te 25-(OH)VD i runga i te kiriuhi. Ko te kukū o te 25-(OH)VD he hononga kino mō te tohu fluorescence, ā, ka taea te kite i te kukū o te 25-(OH)VD i roto i te tauira mā te whakamātautau immunoassay fluorescence.

Tukanga Whakamātautau:

Pānuihia te pukapuka whakahaere taputapu me te tāpiringa ki te kete i mua i te whakamātautau.

1. Waiho ngā matū me ngā tauira katoa ki te pāmahana rūma.
2. Tangohia te kāri whakamātautau i te putea pepa konutea.
3. Tāpirihia he tauira toto/plasma/toto katoa/matimati 0μL ki roto i te otinga, ka whakaohokia kia pai.
4. Pānuihia te hua o te whakamātautau i roto i te 15 meneti.