Kete Tātaritanga mōCalprotectin(Whakamātautau Ārai-Kōromātohiko Hikoirangi)
Mō te whakamahinga tātaritanga in vitro anake
Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA
Kete Tātaritanga mōCalprotectinHe pai te whakamātautau Cal (Cal) mō te whakatau ine i ngā paru tangata Cal mā te whakamātautau fluorescence immunochromatographic, he mea nui hei āwhina i te tautuhi i ngā mate puku mumura. Me whakaū te tauira pai katoa mā ētahi atu tikanga. Mō ngā tohunga hauora anake tēnei whakamātautau.

WHAKARĀPOPOTONGA
He heterodimer a Cal, he mea tito mai i te MRP 8 me te MRP 14^[1]Kei roto i te cytoplasm o ngā neutrophils, ā, kei runga i ngā kiriuhi pūtau mononuclear. He pūmua wāhanga whakapeka a Cal, he wāhanga pumau mō te wiki kotahi i roto i ngā paru tangata, e whakatauhia ana he tohu mate puku mumura.^[2-3]He whakamātautau tauanga tirohanga māmā tēnei kete e kitea ai te Cal i roto i ngā paru tangata, he tino aro ki te kite me te tino motuhake. Ko te whakamātautau e hangai ana ki te tino motuhake o te kaupapa tauhohenga paturopi takirua me ngā tikanga tātari whakamātautau fluorescence immunochromatographic, ka taea te whakaputa hua i roto i te 15 meneti.

TE MĀTĀPONO O TE TIKANGA
Kei te rīpene he paturopi paninga anti-Cal kei te rohe whakamātautau, e mau ana ki te chromatography membrane i mua. Ka pania te papa tapanga ki te paturopi anti-Cal kua tohua ki te fluorescence i mua. I te whakamātautau i te tauira pai, ka taea te whakaranu i te Cal i roto i te tauira me te paturopi anti-Cal kua tohua ki te fluorescence, ka hanga he ranunga ārai mate. I te mea ka tukuna te ranunga kia heke i te rīpene whakamātautau, ka hopukina te matatini honohono Cal e te paturopi paninga anti-Cal i runga i te membrane ka hanga he matatini. He hononga pai te kaha o te fluorescence ki te ihirangi Cal. Ka taea te kite i te Cal i roto i te tauira mā te tātari immunoassay fluorescence.

NGĀ RĀWENI ME NGĀ RAUEMI I TUKUNA

Ngā wāhanga mōkihi 25T：
Kāri whakamātautau takitahi, he mea takai ki te pepa konutea me te whakamaroke 25T
Ngā wairewa tauira 25T
Tāpiringa mōkihi 1

NGĀ RAUEMI E HIAHIATIA ANA ENGARI KĀORE I HOATU
Ipu kohinga tauira, taima

TE KOHINGA TAUIRA ME TE ROKIROKI
1. Whakamahia he ipu ma, ka taea te whakamahi hei kohi i ngā tauira paru hou, ka whakamātauria tonutia. Ki te kore e taea te whakamātau tonutia, rongoatia ki te 2-8°C mō ngā rā e 3, ki raro iho rānei i te -15°C mō ngā marama e 6.
2. Tangohia te rākau tauira kua whakaurua ki roto i te tauira paru, ka mahi anō i te mahi kia toru ngā wā, ka tangohia ngā wāhanga rerekē o te tauira paru i ia wā, kātahi ka whakahokia te rākau tauira, ka whakapirihia kia mau, ka rūrū pai. Mā te whakamahi rānei i te rākau tauira, ka kōwhiria te tauira paru kia 50mg pea, ka whakauru ki roto i te ngongo tauira paru kei roto te waimeha tauira, ka whakapirihia kia mau.
3. Whakamahia he pipette hei tango tauira paru mai i te tūroro mate korere, kātahi ka tāpirihia kia 3 ngā pata (tata ki te 100µL) ki te ngongo tauira paru ka rūrū kia pai.

Ngā Tuhipoka:
1. Āta karohia ngā huringa tio-rewa.
2. Whakarewahia ngā tauira ki te pāmahana rūma i mua i te whakamahinga.

TE TIKANGA WHAKAMĀTAUTAU

Pānuihia te pukapuka whakahaere taputapu me te tāpiringa ki te kete i mua i te whakamātautau.
1. Waiho ngā matū me ngā tauira katoa ki te pāmahana rūma.
2. Whakatuwheratia te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101), tāuruhia te kupuhipa takiuru pūkete e ai ki te tikanga whakahaere o te taputapu, ka tomo ki te atanga kimi.
3. Matawaihia te waehere whakaū niho hei whakaū i te tūemi whakamātautau.
4. Tangohia te kāri whakamātautau i te putea pepa konutea.
5. Whakauruhia te kāri whakamātautau ki roto i te mokamoka kāri, matawaihia te waehere QR, ka kimihia te tūemi whakamātautau.
6. Tangohia te taupoki i te ngongo tauira ka maka atu ngā pata tuatahi e rua o te tauira kua whakaranua, tāpirihia kia 3 ngā pata (tata ki te 100µL) o te tauira kua whakaranua kia poutū, ka ringihia mārire ki roto i te poka tauira o te kāri me te ipu kua whakaratohia.
7. Pāwhiritia te pātene "whakamātautau paerewa", i muri i te 15 meneti, ka kitea aunoa e te taputapu te kāri whakamātautau, ka taea te pānui i ngā hua mai i te mata whakaatu o te taputapu, ā, ka tuhia/tāia ngā hua whakamātautau.
8. Tirohia ngā tohutohu mō te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101).

NGĀ UARA E TŪMANAKOHIA ANA
Karori <60μg/g
E taunakihia ana kia whakatūhia e ia taiwhanga tōna ake awhe noa e tohu ana i te taupori tūroro.

NGĀ HUA WHAKAMĀTAUTAU ME TE WHAKAMĀRAMATANGA
1. He nui ake i te 60μg/g te Calorie i roto i te tauira, ā, me whakakore te panoni o te āhua ā-tinana. He tino rerekē ngā hua, ā, me tātaritanga me ngā tohu haumanu.
2. Ka pā noa ngā hua o tēnei tikanga ki ngā awhe tohutoro kua whakatūria i roto i tēnei tikanga, ā, kāore he whakataurite tika ki ētahi atu tikanga.
3. Ka taea hoki e ētahi atu āhuatanga te whakaputa hapa i ngā hua kimi, tae atu ki ngā take hangarau, ngā hapa whakahaere me ētahi atu āhuatanga tauira.

TE ROKIROKI ME TE PŪMAU
1. E 18 marama te roa o te oranga o te kete mai i te rā i hangaia ai. Penapenahia ngā kete kāore anō kia whakamahia ki te 2-30°C. KAUA E WHAKATIO. Kaua e whakamahia i tua atu i te rā paunga.
2. Kaua e huakina te putea hiri kia rite rā anō koe ki te mahi i tētahi whakamātautau, ā, e taunakihia ana kia whakamahia te whakamātautau kotahi-whakamahinga i raro i te taiao e hiahiatia ana (pāmahana 2-35℃, haumākū 40-90%) i roto i te 60 meneti i te wā tere rawa atu.
3. Ka whakamahia te wai whakamātao tauira i muri tonu i te whakatuwheratanga.

NGĀ WHAKATŪPATO ME NGĀ WHAKATŪPATO
Me hiri te kete, ā, me tiaki hoki i te makuku.
Me whakamana ngā tauira pai katoa mā ētahi atu tikanga.
Me whakaaro ngā tauira katoa he poke pea.
KAUA e whakamahia te matū kua pau te wā.
.KAUA e whakawhiti i ngā matū matū i waenga i ngā kete he rerekē te nama puranga.
KAUA e whakamahi anō i ngā kāri whakamātautau me ngā taputapu whakakorekore.
.Mēnā he te whakamahinga, he nui rawa, he iti rānei te tauira, ka rerekē pea ngā hua.

WHAKAWHĀITI
Pērā i ngā whakamātautau katoa e whakamahi ana i ngā paturopi kiore, tera pea ka pāngia te tauira e ngā paturopi ārai-kiore tangata (HAMA). Tērā pea kei roto i ngā tauira mai i ngā tūroro kua whiwhi i ngā whakaritenga o ngā paturopi monoclonal mō te tātaritanga, te rongoā rānei, te HAMA. Tērā pea ka puta he hua hē, he hua hē rānei.
Hei tohutoro haumanu anake tēnei hua whakamātautau, kaua e waiho hei pūtake anake mō te tātaritanga haumanu me te maimoatanga, me whai whakaaro whānui te whakahaere haumanu o ngā tūroro me ōna tohu, hītori hauora, ētahi atu whakamātautau taiwhanga, urupare maimoatanga, mate urutā me ētahi atu mōhiohio.
Ka whakamahia anake tēnei matū hei whakamātautau paru. Tērā pea kāore e tika te hua ina whakamahia mō ētahi atu tauira pēnei i te huware me te mimi me ētahi atu.

NGĀ ĀHUATANGA MAHI

NGĀ TOHUTORO
1.Li,G.&Y.L.Li.Te whanaungatanga i waenga i te konupūmā me te mate haumanu[J].The Journal of Practical Medicine,2007,23(15)
2.Han,W.,Xu,JM,et al. Te rangahau haumanu o te calamine me te lactoferrin i roto i ngā tūroro e pangia ana e te ulcerative colitis [J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB,et al. Te rangahau mō te whanaungatanga i waenga i te calamine paru me te mate mumura o te puku [J]. Pūtaiao Hangarau me te Hangarau,2010-03,10(8)

Ngā tohu matua i whakamahia:

	Pūrere Hauora Tātaritanga In Vitro
	Kaihanga
	Penapena ki te 2-30℃
	Rā Paunga
	Kaua e Whakamahi Anō
	WHAKATŪPATO
	Tirohia ngā Tohutohu Whakamahinga

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