Ko te nuinga o te Tf kei roto i te toto toto, ko te ihirangi toharite he 1.20~3.25g/L. I roto i ngā paru o te tangata hauora, tata kore rawa he kitea. Ina toto te ara kai, ka rere te Tf i roto i te toto toto ki roto i te ara kai ka tukuna atu me ngā paru, ka nui i roto i ngā paru o ngā tūroro toto puku. Nō reira, he mea nui te mahi a te Tf paru hei kimi i te toto puku. He whakamātautau tirohanga māmā tēnei kete e kitea ai te Tf i roto i ngā paru tangata, he tino aro ki te kimi me te tino motuhake. Ko te whakamātautau e hangai ana ki te kaupapa tauhohenga paturopi takirua tino motuhake me ngā tikanga tātari whakamātautau immunochromatographic koura, ka taea te whakaputa hua i roto i te 15 meneti.