IHAME

Agace k'igikoresho cyo gupima gatwikiriwe n'agace ka BSA na 25-(OH)VD ku gace k'igeragezwa n'agace ka anti-inkwavu ka IgG ku gace kagenzurwa. Agapapuro k'ikarita gatwikiriwe n'agace ka anti-inkwavu ka 25-(OH)VD kari ku gace kagenzurwa mbere y'igihe. Mu gihe cyo gupima icyitegererezo, 25-(OH)VD mu gipimo ihuzwa n'agace ka anti-inkwavu ka 25-(OH)VD kari ku gace kagenzurwa, maze bigakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'igikorwa cya immunochromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo agace kanyuze mu gace kagenzurwa, ikimenyetso cya fluorescent cyigenga kizahuzwa na 25-(OH)VD ku gace kagenzurwa. Igipimo cya 25-(OH)VD ni isano iri hagati y'ikimenyetso cya fluorescence, kandi igipimo cya 25-(OH)VD mu gipimo gishobora kumenyekana hakoreshejwe isuzuma ry'ubudahangarwa bw'umubiri.

Uburyo bwo gupima:

Mbere yo gupima, soma igitabo cy'amabwiriza agenga imikorere y'igikoresho n'agasanduku k'ibikoresho.

1. Shyira ku ruhande ibikoresho byose bitanga ubushyuhe n'ibipimo ku bushyuhe bw'icyumba.
2. Kura ikarita yo gupima mu gafuka k'ifishi.
3. Shyiramo 0μL mu gipimo cya serum/plasma/amaraso yose/urutoki, hanyuma uvange neza
4. Soma ibisubizo by'ikizamini mu minota 15.