Ikhithi yokuxilonga ye-Antibody Subtype ukuya kwi-Helicobacter Pylori
Ulwazi lwemveliso
| Inombolo yoMzekelo | I-HP-ab-s | Ukupakisha | Iimvavanyo ezingama-25/ikhithi, iikhithi ezingama-30/i-CTN |
| Igama | Uhlobo lwe-Antibody Subtype kwiHelicobacter Pylori | Ukuhlelwa kwezixhobo | Iklasi yoku-1 |
| Iimbonakalo | Uvakalelo oluphezulu, Ukusebenza lula | Isatifikethi | I-CE/ ISO13485 |
| Ukuchaneka | > 99% | Beka ubomi kwishelufa | Iminyaka emibini |
| Indlela yokusebenza | Uvavanyo lwe-Fluorescence Immunochromatographic | Inkonzo ye-OEM/ODM | Iyafumaneka |
Isishwankathelo
I-Helicobacter pylori ziibhaktheriya ezingenayo igram, kwaye imo yokugoba kwe-spiral iyinika igama le-helicobacterpylori. I-Helicobacter pylori ihlala kwiindawo ezahlukeneyo zesisu kunye ne-duodenum, nto leyo eya kukhokelela ekuvuvukeni okungapheliyo kwe-mucosa yesisu, izilonda zesisu kunye ne-duodenal, kunye nomhlaza wesisu. I-International Agency for Research on Cancer ichonge usulelo lwe-HP njenge-Class I carcinogen ngo-1994, kwaye i-HP ebangela umhlaza ikakhulu iqulethe ii-cytotoxins ezimbini: enye yi-cytotoxin-associated CagA protein, enye yi-vacuolating cytotoxin (VacA). I-HP inokwahlulwa ibe ziintlobo ezimbini ngokusekelwe ekubonakalisweni kwe-CagA kunye ne-VacA: uhlobo I luhlobo lwe-toxigenic (ngokubonakaliswa kwe-CagA kunye ne-VacA okanye nayiphi na enye yazo), olubangela izifo ezininzi kwaye kulula ukubangela izifo zesisu; uhlobo II luyi-HP ye-atoxigenic (ngaphandle kokubonakaliswa kwe-CagA kunye ne-VacA), engenabungozi kangako kwaye idla ngokungabi nazimpawu zonyango xa usulelo.
Uphawu:
• Uvakalelo oluphezulu
• ukufundwa kweziphumo kwimizuzu eli-15
• Ukusebenza okulula
• Ixabiso elithe ngqo lefektri
• kufuneka umatshini wokufunda iziphumo
Injongo Yokusebenzisa
Le khithi iyasebenza ekufumaneni i-antibody ye-Urease, i-antibody ye-CagA kunye ne-antibody ye-VacA kwi-helicobacter pylori egazini lomntu, kwisampulu yeserum okanye kwiplasma, kwaye ifanelekile ekuxilongweni kosulelo lwe-HP kunye nokuchongwa kohlobo lwesigulana esine-helicobacter pylori. Le khithi inika kuphela iziphumo zovavanyo lwe-antibody ye-Urease, i-antibody ye-CagA kunye ne-antibody ye-VacA kwi-helicobacter pylori, kwaye iziphumo ezifunyenweyo mazisetyenziswe kunye nolunye ulwazi lweklinikhi ukuze kuhlalutywe. Kufuneka isetyenziswe kuphela ziingcali zempilo.
Inkqubo yovavanyo
| 1 | I-1: Ukusetyenziswa kwe-analyzer ye-immune ephathekayo |
| 2 | Vula iphakheji ye-reagent yebhegi yefoyile ye-aluminiyam uze ukhuphe isixhobo sovavanyo. |
| 3 | Faka isixhobo sovavanyo ngokuthe tye kwindawo yokuhlaziya umzimba. |
| 4 | Kwiphepha lasekhaya le-interface yokusebenza ye-immune analyzer, cofa u-“Standard” ukuze ufake i-interface yovavanyo. |
| 5 | Cofa u-“QC Scan” ukuze uskene ikhowudi ye-QR kwicala langaphakathi lekhithi; iiparameter ezinxulumene nekhithi yokufaka kwisixhobo kwaye ukhethe uhlobo lwesampulu. Qaphela: Inani ngalinye lebhetshi yekhithi kufuneka liskene kube kanye. Ukuba inombolo yebhetshi iskene, ngoko tsiba eli nyathelo. |
| 6 | Jonga ukuhambelana kwe "Igama leMveliso", "Inombolo yeBatch" njl. njl. kwi-interface yovavanyo kunye nolwazi olukwileyibhile yekhithi. |
| 7 | Qalisa ukongeza isampuli xa ulwazi luhambelana:Inyathelo 1: thatha isampuli yegazi elipheleleyo eliyi-80μL kancinci kancinci, uze ungazijongi iibhamuza zegazi eliphantsi; Inyathelo lesi-2: isampulu yepipette ukuya kwisampulu ye-diluent, uze udibanise kakuhle isampulu nesampulu ye-diluent; Inyathelo lesi-3: i-pipette engama-80µL ixutywe kakuhle kwisixhobo sovavanyo, kwaye qaphela ukuba akukho maqamza e-pipette ngexesha lokuthatha isampuli |
| 8 | Emva kokongeza isampuli epheleleyo, cofa u-“Timing” kwaye ixesha eliseleyo lovavanyo liya kuboniswa ngokuzenzekelayo kwi-interface. |
| 9 | I-immune analyzer izakugqiba uvavanyo kunye nohlalutyo ngokuzenzekelayo xa ixesha lovavanyo lifikile. |
| 10 | Emva kokuba uvavanyo lwe-immune analyzer lugqityiwe, iziphumo zovavanyo ziya kuboniswa kwi-interface yovavanyo okanye zinokujongwa "kwiMbali" kwiphepha lasekhaya le-interface yokusebenza. |
Umboniso
