Ikhithi yokuxilonga yeMyoglobin (uvavanyo lwe-fluorescence immunochromatographic)

Ukusetyenziswa kokuxilongwa kwi-vitro kuphela

Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO

Ikhithi yokuxilonga ye-myoglobin (uvavanyo lwe-fluorescence immunochromatographic) luvavanyo lwe-fluorescence immunochromatographic lokufumanisa ubungakanani kunye noxinzelelo lwe-myoglobin (MYO) kwi-serum okanye kwi-plasma yomntu, esetyenziswa kakhulu njengoncedo ekuxilongweni kwe-acute myocardial infarction. Olu vavanyo lwenzelwe ukusetyenziswa kobuchwephesha bezempilo kunye nokusetyenziswa kobuchwephesha basekhaya kuphela.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-MYO antibody kwindawo yovavanyo kunye ne-anti-rabbit IgG antibody yebhokhwe kwindawo yokulawula. I-Lable pad igqunywe nge-fluorescence ebhalwe nge-anti-MYO antibody kunye ne-rabbit IgG kwangaphambili. Xa kuvavanywa isampuli, i-MYO antigen kwisampuli idibana ne-anti-MYO antibody ebhalwe nge-fluorescence, kwaye yenze umxube womzimba. Phantsi kwesenzo se-immunochromatography, ukuhamba kwe-complex kwicala lephepha elifunxayo. Xa i-complex idlule kwindawo yovavanyo, idibene ne-anti-MYO coating antibody, yenza i-complex entsha. Inqanaba le-MYO lihambelana kakuhle nesignali ye-fluorescence, kwaye uxinzelelo lwe-MYO kwisampuli lunokufunyanwa ngovavanyo lwe-fluorescence immunoassay.