Ikhithi Yokuxilonga(I-1965)Igolide le-Colloidal）i-Antibody ye-Helicobacter Pylori
Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela
Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi Yokuxilonga (i-Colloidal gold) ye-Antibody to Helicobacter Pylori ifaneleka ukuthola i-antibody ye-HP esezingeni eliphezulu egazini lomuntu, e-serum noma kumasampula e-plasma. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela. Le reagent isetshenziselwa ukusiza ekuxilongweni kokutheleleka kwe-helicobacter pylori esiswini.

USAYIZI WEPAKETHE
Ikhithi eli-1 /ibhokisi, amakhithi ayi-10 /ibhokisi, amakhithi angama-25, /ibhokisi, amakhithi angama-50 /ibhokisi.

ISIFINYEZO
Ukutheleleka kwe-Helicobacter pylori kanye ne-gastritis engapheli, isilonda sesisu, i-gastric adenocarcinoma, i-gastric mucosa associated lymphoma inobuhlobo obuseduze, ku-gastritis, isilonda sesisu, isilonda se-duodenal kanye nomdlavuza wesisu ezigulini ezinezinga lokutheleleka kwe-HP elingaba ngu-90%. Inhlangano yezempilo yomhlaba ibeke i-HP ohlwini njengohlobo lokuqala lwe-carcinogen, kanye nezici ezithile eziyingozi zomdlavuza wesisu. Ukutholwa kwe-HP kuwukuxilongwa kokutheleleka kwe-HP.^[1]. Le khithi iwukuhlolwa okulula, okubonakalayo okulinganiselwe okuthola i-HP kumasampula egazi lomuntu, i-serum noma i-plasma, inokuzwela okuphezulu kokuthola kanye nokucaciswa okuqinile. Le khithi isekelwe kubuchwepheshe bokuhlaziya i-colloidal gold immune chromatography ekutholeni i-antibody ye-HP kumasampula egazi lonke, i-serum noma i-plasma, okunganikeza umphumela zingakapheli imizuzu eyi-15.

INQUBO YOKUHLOLA
1 Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni elilinganayo bese ulimaka.
2 Ukwengeza isampula:
I-Serum ne-plasma: engeza amaconsi amabili e-serum ne-plasma embobeni yesampula engeziwe ene-plastic drip, bese ufaka i-1 drop sampuli diluent, qala isikhathi.
Igazi eliphelele: engeza amaconsi amathathu esampula legazi eliphelele emgodini wesampula nge-drip yepulasitiki, bese ufaka i-diluent yesampula eli-1, qala isikhathi.
Igazi eliphelele elisesinqeni somunwe: engeza amaconsi angu-75µL noma amaconsi amathathu egazi eliphelele elisesinqeni somunwe emgodini wesampula nge-drip yepulasitiki, bese ufaka i-diluent yesampula eli-1, qala isikhathi.
3. Umphumela kufanele ufundwe zingakapheli imizuzu eyi-10-15, futhi awusebenzi ngemva kwemizuzu eyi-15.