ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-conjugate ye-BSA kanye ne-25-(OH)VD esifundeni sokuhlola kanye ne-antibody yembuzi yokulwa nonogwaja IgG esifundeni sokulawula. Iphedi yomaka imbozwe nge-antibody ye-fluorescence mark anti 25-(OH)VD kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula, i-25-(OH)VD kusampula ihlangana ne-antibody ye-antibody ye-fluorescence mark anti 25-(OH)VD, bese yakha ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula esifundeni sokuhlola, i-free fluorescent marker izohlanganiswa ne-25-(OH)VD ku-membrane. Ukuhlushwa kwe-25-(OH)VD kuwukuxhumana okungekuhle kwesignali ye-fluorescence, futhi ukuhlushwa kwe-25-(OH)VD kusampula kungatholakala nge-fluorescence immunoassay assay.

Inqubo Yokuhlola:

Sicela ufunde incwadi yemiyalelo yokusebenza kwethuluzi kanye nephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagent namasampula endaweni yokushisa yegumbi.
2. Khipha ikhadi lokuhlola esikhwameni se-foil.
3. Faka isampula ye-serum/plasma/igazi eliphelele/iminwe engu-0μL esixazululweni, bese uxuba kahle
4. Funda umphumela wokuhlolwa zingakapheli imizuzu eyi-15.