ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-anti-HbA1c antibody endaweni yokuhlola kanye ne-anti-Hb antibody endaweni yokulawula.Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi i-anti-Hb antibody kanye ne-anti-HbA1c antibody kusengaphambili.Lapho kuhlolwa isampula, i-antigen ye-HbA1c kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti-HbA1c antibody, i-antigen ye-Hb esesampuli ihlanganisa ne-fluorescence enelebuli ye-anti Hb antibody futhi yakhe ingxube yamasosha omzimba.Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlangene ne-anti-HbA1c yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha.lapho inkimbinkimbi idlula isifunda sokulawula, ihlangene ne-anti-Hb coating antibody, yakha inkimbinkimbi entsha.Izinga le-HbA1c lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukugxila kwe-HbA1c ne-Hb kusampula kungatholwa nge-fluorescence immunoassay assay.

Inqubo Yokuhlola:

Sicela ufunde imanuwali yokusebenza kwezinsimbi kanye nokufakwa kwephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2. Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yensimbi, bese ufaka isixhumi esibonakalayo sokubona.
3. Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5. Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola
6. Faka i-10μL Igazi eliphelele kwisampula ye-diluent, nyakazisa iminithi elingu-1 bese uxuba kahle.
7. Engeza isisombululo sesampula esingu-80μL ukuze uthole isampula yomthombo wekhadi.
8. Chofoza inkinobho ethi "standard test", ngemva kwemizuzu engu-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode/liphrinte imiphumela yokuhlolwa.
9. Bheka imiyalelo ye-Portable Immune Analyzer(WIZ-A101).