Ikhithi Yokuxilonga Yohlobo Olungaphansi Lomzimba Womzimba ku-Helicobacter Pylori
Ulwazi lokukhiqiza
| Inombolo Yemodeli | I-HP-ab-s | Ukupakisha | Izivivinyo ezingu-25/ikhithi, amakhithi angu-30/i-CTN |
| Igama | Uhlobo Oluncane Lokulwa Nomzimba Ku-Helicobacter Pylori | Ukuhlukaniswa kwezinsimbi | Iklasi I |
| Izici | Ukuzwela okuphezulu, Ukusebenza okulula | Isitifiketi | I-CE/ ISO13485 |
| Ukunemba | > 99% | Isikhathi sokuphelelwa yisikhathi | Iminyaka Emibili |
| Indlela Yokwenza | Ukuhlolwa kwe-Fluorescence Immunochromatographic | Isevisi ye-OEM/ODM | Kuyatholakala |
Isifinyezo
I-Helicobacter pylori yibhaktheriya engenalo i-gram, futhi ukuma kokugoba okujikelezayo kuyinika igama le-helicobacterpylori. I-Helicobacter pylori ihlala ezindaweni ezahlukene zesisu kanye ne-duodenum, okuzoholela ekuvuvukeni okuncane okungapheli kwe-mucosa yesisu, izilonda zesisu kanye ne-duodenal, kanye nomdlavuza wesisu. I-International Agency for Research on Cancer yathola ukutheleleka kwe-HP njenge-carcinogen ye-Class I ngo-1994, kanti i-HP ebangela umdlavuza ikakhulukazi iqukethe ama-cytotoxin amabili: enye iyiprotheni ye-CagA ehlobene ne-cytotoxin, enye iyi-cytotoxin ekhipha i-vacuolating (VacA). I-HP ingahlukaniswa ngezinhlobo ezimbili ngokusekelwe ekubonakalisweni kwe-CagA kanye ne-VacA: uhlobo I uhlobo lwe-toxigenic (ngokubonakaliswa kwe-CagA kanye ne-VacA noma enye yazo), olubangela izifo eziningi futhi olubangela izifo zesisu; uhlobo II luyi-HP engachazi i-toxigenic (ngaphandle kokubonakaliswa kwe-CagA kanye ne-VacA), olungenabo ubuthi obuncane futhi ngokuvamile alunazimpawu zomtholampilo lapho lutheleleka.
Isici:
• Ukuzwela okuphezulu
• ukufundwa kwemiphumela ngemizuzu eyi-15
• Ukusebenza okulula
• Intengo eqondile yasefektri
• udinga umshini wokufunda imiphumela
Sebenzisa Ngokuhlosile
Le khithi iyasebenza ekutholakaleni kwe-antibody ye-Urease, i-antibody ye-CagA kanye ne-antibody ye-VacA ku-helicobacter pylori egazini lomuntu lonke, isampula ye-serum noma ye-plasma, futhi ifanelekela ukuxilongwa okusizayo kokutheleleka kwe-HP kanye nokuhlonza uhlobo lwesiguli se-helicobacter pylori esitheleleke ngaso. Le khithi inikeza kuphela imiphumela yokuhlola ye-antibody ye-Urease, i-antibody ye-CagA kanye ne-antibody ye-VacA ku-helicobacter pylori, futhi imiphumela etholakele kufanele isetshenziswe kanye nolunye ulwazi lwezokwelapha ukuze luhlaziywe. Kumele isetshenziswe ochwepheshe bezempilo kuphela.
Inqubo yokuhlola
| 1 | I-1: Ukusetshenziswa kwe-analyzer yokuzivikela yomzimba ephathekayo |
| 2 | Vula iphakethe lesikhwama se-aluminium foil eline-reagent bese ukhipha idivayisi yokuhlola. |
| 3 | Faka idivayisi yokuhlola ngokuvundlile endaweni yokuhlaziya amasosha omzimba. |
| 4 | Ekhasini lasekhaya le-interface yokusebenza ye-immune analyzer, chofoza okuthi “Standard” ukuze ufake i-interface yokuhlola. |
| 5 | Chofoza okuthi “QC Scan” ukuze uskene ikhodi ye-QR ngaphakathi kwekhithi; amapharamitha ahlobene nekhithi yokufaka kuthuluzi bese ukhetha uhlobo lwesampula. Qaphela: Inombolo ngayinye yebhetshi yekhithi kufanele iskene kanye. Uma inombolo yebhetshi isiskene, khona-ke yeqa lesi sinyathelo. |
| 6 | Hlola ukuvumelana “kwegama lomkhiqizo”, “inombolo yeqoqo” njll. esibonakalayo sokuhlola ngolwazi oluselebula lekhithi. |
| 7 | Qala ukwengeza isampula uma ulwazi luhambisana:Isinyathelo 1: thatha isampula yegazi eliphelele eliyi-80μL kancane kancane, bese uqaphela ukuthi awekho amabhamuza e-pipette; Isinyathelo 2: isampula ye-pipette ukuze isampula i-diluent, bese uxuba kahle isampula nesampula i-diluent; Isinyathelo 3: isixazululo se-pipette esingu-80µL sixutshwe kahle emthonjeni wedivayisi yokuhlola, bese unaka amabhamuza e-pipette ngesikhathi sokuthatha amasampula |
| 8 | Ngemva kokwengeza okuphelele kwesampula, chofoza okuthi “Isikhathi” bese isikhathi esisele sokuhlola sizoboniswa ngokuzenzakalelayo ku-interface. |
| 9 | I-immune analyzer izoqedela ngokuzenzakalelayo ukuhlolwa nokuhlaziywa lapho isikhathi sokuhlolwa sesifikile. |
| 10 | Ngemva kokuthi ukuhlolwa yi-immune analyzer sekuqediwe, umphumela wokuhlolwa uzoboniswa ku-interface yokuhlola noma ungabukwa "ngomlando" ekhasini lasekhaya le-interface yokusebenza. |
Umbukiso
