ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-ALB antigen endaweni yokuhlola kanye ne-antibody yembuzi yokulwa nonogwaja IgG endaweni yokulawula. Iphedi yomaka imbozwe nge-antibody ye-ALB uphawu lwe-fluorescence kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula, i-ALB kusampula ihlangana ne-antibody ye-ALB uphawu lwe-fluorescence, bese yakha ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula endaweni yokuhlola, i-free fluorescent marker izohlanganiswa ne-ALB ku-membrane. Ukuhlushwa kwe-ALB kuwukuxhumana okungekuhle kwesignali ye-fluorescence, futhi ukuhlushwa kwe-ALB kusampula kungatholakala nge-fluorescence immunoassay assay.

Inqubo Yokuhlola:

Sicela ufunde incwadi yemiyalelo yokusebenza kwethuluzi kanye nephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagent namasampula endaweni yokushisa yegumbi.
2. Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3. Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5. Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa.
6. Faka isampula yomchamo engu-80μL emthonjeni wekhadi
7. Chofoza inkinobho ethi "ukuhlolwa okujwayelekile", ngemva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
8. Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).