Ikhithi Yokuxilonga ye-Myoglobin (ukuhlolwa kwe-fluorescence immunochromatographic)

Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela

Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Ikhithi Yokuxilonga ye-myoglobin (i-fluorescence immunochromatographic assay) iyi-fluorescence immunochromatographic assay yokubona inani kanye nokuhlushwa kwe-myoglobin (MYO) ku-serum noma ku-plasma yomuntu, esetshenziswa kakhulu njengosizo ekuxilongweni kwe-acute myocardial infarction. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kanye nokusetshenziswa kochwepheshe basekhaya kuphela.

ISIMISO SENQUBO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-anti-MYO antibody endaweni yokuhlola kanye ne-anti-IgG antibody yembuzi endaweni yokulawula. Iphedi yelebula imbozwe nge-anti-MYO antibody ebhalwe nge-fluorescence kanye ne-IgG yenogwaja kusengaphambili. Lapho kuhlolwa isampula, i-antigen ye-MYO kusampula ihlangana ne-anti-MYO antibody ebhalwe nge-fluorescence, bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo. Lapho i-complex idlula esifundeni sokuhlola, ihlanganiswe ne-anti-MYO coating antibody, yakha i-complex entsha. Izinga le-MYO lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukuhlushwa kwe-MYO kusampula kungatholakala nge-fluorescence immunoassay assay.