TE MATUA

Kua pania te kiriuhi o te taputapu whakamātautau ki te antigen Rotavirus Group A i te rohe whakamātautau me te paturopi IgG anti-rāpeti koati i te rohe whakahaere. Kua pania te papa tapanga ki te tohu fluorescence anti-Rotavirus Group A me te IgG rāpeti i mua. I te whakamātautau i te tauira pai, ka honoa te RV i roto i te tauira ki te tohu fluorescence anti-Rotavirus Group A, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti. Ina paahitia e te matatini te rohe whakamātautau, ka honoa ki te paturopi paninga anti-Rotavirus Group A, ka hanga he matatini hou. Mena he kino, kaore he antigen Rotavirus Group A i roto i te tauira, no reira kaore e taea te hanga matatini ārai mate, kaore he raina whero i te rohe kitenga (T). Ahakoa kei roto i te tauira te rotavirus group A, ka chromatographed te IgG kiore kua tohua ki te rohe whakahaere kounga (C) ka hopukina e te paturopi IgG anti-kiore koati. Ka puta he raina whero i te rohe whakahaere kounga (C). Ko te rārangi whero te paerewa e puta ana i te rohe whakahaere kounga (C) hei whakatau mena he nui ngā tauira, me te mea he noa te tukanga chromatography. Ka whakamahia hoki hei paerewa whakahaere ā-roto mō ngā matū.

Tukanga Whakamātautau:

1. Me kohi ngā tūroro e whakaatu ana i ngā tohumate. E ai ki ngā pūrongo, ko te nuinga o te rotavirus e puta ana i roto i ngā paru o ngā tūroro e pāngia ana e te gastroenteritis ka puta i te 3-5 ngā rā i muri i te tīmatanga o te mate, ā, i te 3-13 ngā rā i muri i te tīmatanga o ngā tohumate. Mena ka kohia te tauira i muri roa i te mate korere, tera pea kāore e ranea te maha o ngā antigens hei puta i te tauhohenga pai.

2. Me kohi ngā tauira ki roto i tētahi ipu ma, maroke, ātete-wai, kāore hoki he hopi horoi me he matū tiaki i roto.

3. Mō ngā tūroro kāore i te mate korere, kaua e iti iho i te 1-2 karamu te rahi o ngā tauira paru i kohia. Mō ngā tūroro e mate korere ana, mēnā he wai te paru, kohia kia 1-2 ml te iti rawa o te wai paru. Mēnā he nui te toto me te hūpē kei roto i te paru, kohia anō te tauira.

4. E taunakihia ana kia whakamātauria tonutia ngā tauira i muri i te kohikohinga, ki te kore me tuku atu ki te taiwhanga i roto i ngā hāora e 6, ā, me rongoa ki te 2-8°C. Mēnā kāore anō kia whakamātauria ngā tauira i roto i ngā hāora e 72, me rongoa ki te pāmahana i raro iho i te -15°C.

5. Whakamahia ngā paru hou hei whakamātautau, me ngā tauira paru kua konatunatua ki te wai whakaranu, ki te wai whakapūkara rānei