Kete Tātaritanga（RATEKI）mō te Rotavirus Rōpū A me te adenovirus
Mō te whakamahinga tātaritanga in vitro anake
Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA
He pai te Kete Tātaritanga (LATEX) mō te Rotavirus Rōpū A me te adenovirus mō te kimi kounga o te Rotavirus Rōpū A me te antigen adenovirus i roto i ngā tauira paru tangata. Mō ngā tohunga hauora anake tēnei whakamātautau. I tēnei wā, ka whakamahia tēnei whakamātautau mō te tātaritanga haumanu o te mate korere pēpi i roto i ngā tūroro e pangia ana e te Rotavirus Rōpū A Rōpū A.huaketo rotame te mate adenovirus.

RAHI O TE MŌKIHI
1 kete/pouaka, 10 kete/pouaka, 25 kete,/pouaka, 50 kete/pouaka

WHAKARĀPOPOTONGA
Kua whakarōpūtia te rotavirus heihuaketo rotate momo o te huaketo exenteral, he āhua porowhita tōna, ā, he 70nm te whānui. Kei roto i te rotavirus he 11 ngā wāhanga o te RNA aho-rua. Ka taea te wehewehe i te rotavirus ki ngā rōpū e whitu (ag) i runga i ngā rerekētanga antigenic me ngā āhuatanga ira. Kua pūrongohia ngā mate tangata o te rōpū A, rōpū B me te rōpū C rotavirus. Ko te Rotavirus Rōpū A te tino take o te gastroenteritis kino i roto i ngā tamariki puta noa i te ao.^[1-2]E 51 ngā momo adenovirus tangata (HAdV), e 6 pea ngā momo momo (A~F) i runga i te ārai mate me te matū koiora.^[3]Ka taea e ngā adenovirus te pangia te manawa, te puku, te kanohi, te pūkahukahu, me te ate, ā, ka horapa te mate urutā. Ko te hunga he pai te ārai mate ka whakawhanake i ngā paturopi ka ora ai rātou anō. Mō ngā tūroro, mō ngā tamariki rānei kua pehia te ārai mate, ka mate pea ngā mate adenovirus.

TE TIKANGA WHAKAMĀTAUTAU
1. Tangohia te rākau tauira kua whakaurua ki roto i te tauira paru, kātahi ka whakahokia te rākau tauira, ka whakapiripiri kia mau, ka rūrū kia pai, ka whakahokia te mahi kia 3 ngā wā. Mā te whakamahi rānei i te rākau tauira, ka kōwhiria te tauira paru kia 50mg pea, ka whakauru ki roto i te ngongo tauira paru kei roto te waimeha tauira, ka whakapiripiri kia mau.
2. Whakamahia he pipette tango tauira, tangohia te tauira paru angiangi mai i te tūroro mate korere, kātahi ka tāpirihia kia 3 ngā pata (tata ki te 100uL) ki te ngongo tauira paru, ka rūrū pai, ka waiho ki tahaki.
3. Tangohia te kāri whakamātautau mai i te putea pepa konutea, whakatakotoria ki runga i te tēpu papatahi, ka tohua.
4. Tangohia te taupoki i te ngongo tauira ka maka atu ngā pata tuatahi e rua o te tauira kua whakaranua, tāpirihia kia 3 ngā pata (tata ki te 100µL) o te tauira kua whakaranua kia poutū, ka ata ringihia ki roto i te poka tauira o te kāri me te putanga kua whakaratohia, tīmatahia te wā.
5. Me pānui te hua i roto i ngā meneti 10-15, ā, kāore e whai mana i muri i ngā meneti 15.