Ka oati tā mātou kamupene ki ngā tāngata katoa mai i ngā taonga o te karaehe tuatahi me te kamupene hoko-muri tino makona. Ka mihi nui mātou ki ā mātou kaihoko auau me ngā kaihoko hou ki te whakauru mai ki a mātou mō te Kete Whakamātautau Whaiaro Kāinga Nikau, he iti te utu mō ngā taputapu taiwhanga hauora Haina.Whakamātautau Tere Kotahi te HipangaKo te Kete Tātari Immunofluorescence Immunoassay, "Ko te Hanga i ngā Otinga Kounga Nui" te whāinga pumau o tā mātou pakihi. Ka whakapau kaha tonu mātou ki te whakatutuki i te whāinga "Ka Pupuri Tonu Mātou i te Tere i te Wā".
Ka oati tā mātou kamupene ki ngā tāngata katoa mai i ngā taonga tino pai me te kamupene whai muri i te hoko e tino makona ana. Ka mihi nui mātou ki ā mātou kaihoko auau me ngā kaihoko hou ki te whakauru mai ki a mātou mōKaitātari Ārai Mate Pūmua Haina, Whakamātautau Tere Kotahi te Hipanga, Nā te kounga pai me te utu whaitake kua mau mai he kiritaki pumau me te ingoa rongonui. Mā te whakarato i ngā 'Otinga Kounga, Ratonga Tino Pai, Utu Whakataetae me te Tukunga Tere', kei te tumanako mātou ki te mahi tahi nui atu me ngā kiritaki o tāwāhi i runga i ngā painga tahi. Ka mahi tahi mātou me te ngākau nui ki te whakapai ake i ā mātou hua me ā mātou ratonga. Ka oati hoki mātou ki te mahi tahi me ngā hoa pakihi ki te whakaara ake i tā mātou mahi tahi ki te taumata teitei ake, me te whakapuaki i te angitu tahi. Nau mai haere mai ki te toro mai ki tā mātou wheketere.
Kete Tātaritanga mō te Estradiol(whakamātautau ārai-ira-whakaahua-whakaahua)
Mō te whakamahinga tātaritanga in vitro anake
Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA
Ko te Kete Tātaritanga mō te Estradiol (whakamātautau immunochromatographic fluorescence) he whakamātautau immunochromatographic fluorescence mō te kimi i te nui o te Estradiol (E2) i roto i te toto tangata, i te plasma rānei, e whakamahia ana hei aromatawai i ngā taumata o te Estradiol. He matū tātaritanga āwhina. Me whakaū ngā tauira pai katoa mā ētahi atu tikanga. Ko tēnei whakamātautau he mea hanga mō te whakamahinga hauora anake.

WHAKARĀPOPOTONGA
Ko te Estradiol (E2) te homoni tino nui me te tino kaha i roto i te estrogen. Ko tōna taumaha ngota he 272.3 D. I te nuinga o te wā, mō ngā wāhine kāore i te hapu, ka tukuna te E2 e ngā pūtau pūkahukahu me ngā pūtau granular me ngā pūtau luteal i te wā e whanake ana te follicular. I te wā e hapu ana, ka tukuna te E2 e te whenua whānau, ko ngā tāne ia ka whakaputaina e ngā testicles. I muri i te urunga o te E2 ki te toto, 1% ki te 3% kāore e herea ki ngā pūmua, 40% e herea ki te globulin here homoni ira tangata (SHBG), ā, ko ētahi e herea ana ki te albumin, ka pākia mai i te ate ki ngā sulfates wairewa-wai, ki ngā esters gluconaldehyde rānei, ā, ka tukuna mai i te mimi. He tohu homoni nui te E2 mō te aromatawai i te mahi ovarian.

TE MĀTĀPONO O TE TIKANGA
Kua pania te kiriuhi o te taputapu whakamātautau ki te hononga o te BSA me te Estradiol i te rohe whakamātautau me te paturopi IgG anti-rāpeti koati i te rohe whakahaere. Kua pania ngā papa tohu ki te tohu fluorescence anti-E2 me te IgG rāpeti i mua. I te whakamātautau i te tauira, ka honoa te E2 i roto i te tauira ki te paturopi anti-E2 kua tohua ki te fluorescence, ā, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti, ina paahitia e te matatini te rohe whakamātautau, ka honoa te tohu fluorescence kore utu ki te Estradiol i runga i te kiriuhi. He hononga kino te kukū o te Estradiol mō te tohu fluorescence, ā, ka taea te kite i te kukū o te Estradiol i roto i te tauira mā te whakamātautau immunoassay fluorescence.

NGĀ RĀWENI ME NGĀ RAUEMI I TUKUNA

Ngā wāhanga mōkihi 25T：
Kāri whakamātautau takitahi he pepa konutea kua kapi ki te whakamaroke 25T
.He otinga 25T
Otinga .B 1
.Whakaurunga mōkihi 1

NGĀ RAUEMI E HIAHIATIA ANA ENGARI KĀORE I HOATU
Ipu kohinga tauira, taima

TE KOHINGA TAUIRA ME TE ROKIROKI
1. Ko ngā tauira i whakamatautauria he toto toto, he toto toto heparin anticoagulant, he toto toto EDTA anticoagulant rānei.
2. E ai ki ngā tikanga paerewa, kohia he tauira. Ka taea te pupuri i te tauira toto, i te tauira plasma rānei ki roto i te pouaka whakamātao i te 2-8℃ mō ngā rā e 7, ā, ka taea te pupuri i te tauira i raro i te -15°C mō ngā marama e 6.
.Kāore ngā tauira katoa e neke i ngā huringa tio-rewa.

TE TIKANGA WHAKAMĀTAUTAU
Tirohia te pukapuka ā-ringa mō te whakamātautau o te taputapu. Ko te tukanga whakamātautau matū koia tēnei:
1. Waiho ngā matū me ngā tauira katoa ki te pāmahana rūma.
2. Whakatuwheratia te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101), tāuruhia te kupuhipa takiuru pūkete e ai ki te tikanga whakahaere o te taputapu, ka tomo ki te atanga kimi.
3. Matawaihia te waehere whakaū niho hei whakaū i te tūemi whakamātautau.
3. Tangohia te kāri whakamātautau i te putea pepa konutea.
4. Whakauruhia te kāri whakamātautau ki roto i te mokamoka kāri, matawaihia te waehere QR, ka kimihia te tūemi whakamātautau.
5. Tāpirihia he 30μL o te tauira toto, o te tauira parāuma rānei ki te otinga A, ka whakaohokia kia pai.
6. Tāpirihia te 20μL o te otinga B ki te ranunga i runga ake nei, ka whakaohokia kia pai.
Waiho te ranunga mō20meneti.
Tāpirihia he ranunga 80μL ki te puna tauira o te kāri.
Pāwhiritia te pātene "whakamātautau paerewa", i muri i te 10 meneti, ka kitea aunoa e te taputapu te kāri whakamātautau, ka taea e ia te pānui i ngā hua mai i te mata whakaatu o te taputapu, ka tuhi/tā i ngā hua whakamātautau.
Tirohia ngā tohutohu mō te Pūtātari Ārai Mate Kawe (WIZ-A101).

NGĀ HUA WHAKAMĀTAUTAU ME TE WHAKAMĀRAMATANGA

Ko ngā raraunga o runga ake nei te wā tohutoro kua whakatūria mō ngā raraunga kitenga o tēnei kete, ā, e kīia ana me whakatū e ia taiwhanga he wā tohutoro mō te hiranga haumanu e tika ana mō te taupori i tēnei rohe.
.He teitei ake te kukū o te Estradiol i te awhe tohutoro, ā, me ārai ngā huringa ā-tinana, ngā urupare ahotea rānei. He pono he rerekē, me whakakotahi te tātaritanga tohu haumanu.
Ko ngā hua o tēnei tikanga ka pā anake ki te awhe tohutoro kua whakatūria e tēnei tikanga, ā, kāore ngā hua e taea te whakarite tika ki ētahi atu tikanga.
Ka taea hoki e ētahi atu āhuatanga te whakaputa hapa i ngā hua kimi, tae atu ki ngā take hangarau, ngā hapa whakahaere me ētahi atu āhuatanga tauira.

TE ROKIROKI ME TE PŪMAU
1. E 18 marama te roa o te oranga o te kete mai i te rā i hangaia ai. Penapenahia ngā kete kāore anō kia whakamahia ki te 2-30°C. KAUA E WHAKATIO. Kaua e whakamahia i tua atu i te rā paunga.
2. Kaua e huakina te putea hiri kia rite rā anō koe ki te mahi i tētahi whakamātautau, ā, e taunakihia ana kia whakamahia te whakamātautau kotahi-whakamahinga i raro i te taiao e hiahiatia ana (pāmahana 2-35℃, haumākū 40-90%) i roto i te 60 meneti i te wā tere rawa atu.
3. Ka whakamahia te wai whakamātao tauira i muri tonu i te whakatuwheratanga.

NGĀ WHAKATŪPATO ME NGĀ WHAKATŪPATO
Me hiri te kete, ā, me tiaki hoki i te makuku.
Me whakamana ngā tauira pai katoa mā ētahi atu tikanga.
Me whakaaro ngā tauira katoa he poke pea.
KAUA e whakamahia te matū kua pau te wā.
.KAUA e whakawhiti i ngā matū i waenga i ngā kete he rerekē te nama rota..
KAUA e whakamahi anō i ngā kāri whakamātautau me ngā taputapu whakakorekore.
.Mēnā he te whakamahinga, he nui rawa, he iti rānei te tauira, ka rerekē pea ngā hua.

LWHAKATAURANGA
Pērā i ngā whakamātautau katoa e whakamahi ana i ngā paturopi kiore, tera pea ka pāngia te tauira e ngā paturopi ārai-kiore tangata (HAMA). Tērā pea kei roto i ngā tauira mai i ngā tūroro kua whiwhi i ngā whakaritenga o ngā paturopi monoclonal mō te tātaritanga, te rongoā rānei, te HAMA. Tērā pea ka puta he hua hē, he hua hē rānei.
Hei tohutoro haumanu anake tēnei hua whakamātautau, kaua e waiho hei pūtake anake mō te tātaritanga haumanu me te maimoatanga, me whai whakaaro whānui te whakahaere haumanu o ngā tūroro me ōna tohu.
.hītori hauora, ētahi atu whakamātautau taiwhanga, urupare ki te maimoatanga, te mate urutā me ētahi atu mōhiohio.
Ka whakamahia anake tēnei matū hei whakamātautau i te toto me te toto toto. Tērā pea kāore e tika te hua ina whakamahia mō ētahi atu tauira pēnei i te huware me te mimi me ētahi atu.
NGĀ ĀHUATANGA MAHI

RNGĀ WHAKAMĀRAMA
1.Hansen JH, et al. Te Whakararuraru HAMA ki ngā Immunoassays Murine Monoclonal Antibody-Based Immunoassays[J].J o Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Te Āhua o ngā Paturopi Heterophilic me te Tūranga i roto i te Whakararuraru Immunoassay[J].J o Clin Immunoassay,1992,15:108-114.

Ngā tohu matua i whakamahia:

	Pūrere Hauora Tātaritanga In Vitro
	Kaihanga
	Penapena ki te 2-30℃
	Rā Paunga
	Kaua e Whakamahi Anō
	WHAKATŪPATO
	Tirohia ngā Tohutohu Whakamahinga

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