MĀTĀPONO

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te antibody anti EV71 i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere.Ka whakakikoruatia te papa tapanga e te fluorescence kua tapaina he antibody anti EV71 me te IgG rapeti i mua.I te wa e whakamatautau ana i te tauira pai, ka hono te antigen EV71 i roto i te tauira me te fluorescence kua tapaina he antibody anti EV71, ka hanga he ranunga aukati.I raro i te mahi a te chromatography, ka rere te matatini ki te huarahi o te pepa absorbent, ka pahemo te uaua ki te rohe whakamatautau, ka honoa me te antibody paninga anti EV71, ka hanga he matatini hou.

Mena he kino, kaore i roto i te tauira te enterovirus 71 IgM antibody, kia kore ai e taea te hanga i te matatini aukati.Karekau he raina whero i te waahi kitea (T).Ahakoa kei te noho te Enterovirus 71 IgM antibody i roto i te tauira, kaore ranei, ko te toenga o te kiore colloidal koura-tapanga kiore anti-tangata IgM monoclonal antibody me te koati anti-kiore IgG antibody kua pania ki te rohe whakahaere kounga (C).Na ka whakawhanake nga agglutinates i te tae i roto i te waahanga whakahaere kounga, ka puta te raina whero ki roto (C).Ko te raina whero te paerewa ka puta i te waahi whakahaere kounga (C) mo te whakawa mena he nui nga tauira me te mea he rite tonu te mahi chromatography.Ka whakamahia ano hei paerewa mana o roto mo nga reagents.

Tikanga Whakamatau:

1.Ko nga tauira i whakamatauria he toto katoa, tae atu ki te toto venous, toto Peripheral ranei.Kaore e taea te penapena toto katoa i muri i te kohi.Me whakamahi ahau i muri tata i te kohi.

2.Ko nga tauira Serum ka kohia aseptically rite ki tikanga paerewa.Kaore e taea te whakamahi i te serum kore-wera.Kaore i te tūtohutia ki te whakamahi i te serum lipemic, turbid, poke ranei.He matūriki i roto i te serum.A ka pa te ua ki nga hua o te whakamatautau, me whakamaarama, me taraihia ranei nga tauira i mua i te whakamahi.

3.Ko nga tauira i whakamatauhia he heparin, Sodium citrate or EDTA anticoagulant plasma.

4.E ai ki nga tikanga paerewa kohikohi tauira.Ka taea te pupuri i te serum, te tauira plasma ranei i roto i te pouaka whakamātao ki te 2-8 ℃ mo nga ra e 3 me te cryopreservation i raro i te -15°C mo te 3 marama.

5.Katoa nga tauira karo i nga huringa whakatio-rewa.