Ụkpụrụ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale na mgbochi EV71 na mpaghara ule yana mgbochi ewu mgbochi oke bekee IgG na mpaghara njikwa.A na-ekpuchi akwa akwa akwa site na fluorescence akpọrọ mgbochi EV71 mgbochi na oke bekee IgG tupu oge eruo.Mgbe a na-anwale nlele dị mma, antigen EV71 dị na sample jikọtara ya na fluorescence akpọrọ mgbochi EV71, wee mepụta ngwakọta mgbochi.N'okpuru omume nke chromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, ọ jikọtara na mgbochi EV71 mkpuchi mgbochi, na-etolite ọhụrụ mgbagwoju.

Ọ bụrụ na ọ na-adịghị mma, ihe nlele ahụ enweghị ihe mgbochi enterovirus 71 IgM, nke mere na enweghi ike ịmepụta mgbagwoju anya.Agaghị enwe ahịrị uhie na mpaghara nchọpụta (T).N'agbanyeghị ma ọgwụ nje Enterovirus 71 IgM dị na ụdịdị ahụ ma ọ bụ na ọ bụghị, ihe fọdụrụ colloidal gold akara òké mgbochi mmadụ IgM monoclonal antibody na ewu mgbochi òké IgG mgbochi mkpuchi na mpaghara njikwa mma (C).Mgbe ahụ agglutinates na-etolite agba na mpaghara njikwa mma, na ahịrị uhie ga-apụta na (C).Ahịrị uhie bụ ọkọlọtọ na-egosi na mpaghara njikwa mma (C) maka ikpebi ma enwere ihe nlele zuru oke yana ma usoro chromatography ọ bụ ihe nkịtị.A na-ejikwa ya dị ka ụkpụrụ njikwa ime maka reagents.

Usoro nnwale:

1.The samples nwalere nwere ike ịbụ dum ọbara, gụnyere venous ọbara ma ọ bụ Peripheral ọbara.Enweghị ike ịchekwa ọbara zuru oke ma ọ gbakọtachara.Ekwesịrị iji m mee ihe ngwa ngwa ka anakọtachara.

2.Serum samples na-anakọta aseptically dị ka ọkọlọtọ usoro.Ọbara enweghị ọkụ enweghị ike iji.A naghị atụ aro ka iji lipemic, turbid ma ọ bụ serum emetọọ.Tinye ihe na serum.Na mmiri ozuzo ga-emetụta nsonaazụ ule, ihe nlele ndị dị otú ahụ kwesịrị ịbụ centrifuged ma ọ bụ nzacha tupu ejiri ya.

3.The samples nwalere nwere ike ịbụ heparin, Sodium citrate ma ọ bụ EDTA anticoagulant plasma.

4.According ọkọlọtọ usoro na-anakọta sample.Enwere ike idowe ihe nlele ọbara ma ọ bụ plasma refrigerated na 2-8 ℃ maka ụbọchị 3 na cryopreservation n'okpuru -15 Celsius maka ọnwa 3.

5.All sample zere ifriizi-thaw cycles.