ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-anti EV71 antibody endaweni yokuhlola kanye ne-anti-rabbit IgG antibody yembuzi endaweni yokulawula.Iphedi elebulayo limbozwe nge-fluorescence ebhalwe ukuthi i-anti EV71 antibody kanye ne-rabbit IgG kusengaphambili.Lapho kuhlolwa isampula elihle, i-EV71 antigen kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti EV71 antibody, bese yenza ingxube yamasosha omzimba.Ngaphansi kwesenzo se-chromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti EV71 yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha.

Uma inegethivu, isampula alinawo ama-antibody e-enterovirus 71 IgM, ukuze i-immune complex ingakwazi ukwakheka.Ngeke kube khona umugqa obomvu endaweni yokuthola (T).Kungakhathaliseki ukuthi i-Enterovirus 71 IgM antibody ikhona esibonelweni noma cha, igundane elisele elibhalwe ngegolide eline-colloidal anti-human IgM monoclonal antibody kanye ne-anti-mouse IgG antibody ehlanganiswe endaweni yokulawula ikhwalithi (C) iyabopha.Khona-ke ama-agglutinates athuthukisa umbala endaweni yokulawula ikhwalithi, futhi umugqa obomvu uzovela ku-(C).Umugqa obomvu uyindinganiso evela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini.Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo Yokuhlola:

1.Amasampula ahloliwe angaba yigazi eliphelele, okuhlanganisa igazi le-venous noma igazi le-Peripheral.Igazi eliphelele alikwazi ukugcinwa ngemva kokuqoqwa.Kufanele ngisetshenziswe ngokushesha ngemva kokuqoqa.

Amasampula e-2.Serum aqoqwa ngokwe-aseptically ngokuya ngamasu ajwayelekile.I-serum engasebenzi kahle ayikwazi ukusetshenziswa.Akunconywa ukusebenzisa i-lipemic, i-turbid noma i-serum engcolile.Ingxenye ye-particle ku-serum.Futhi imvula izothinta imiphumela yokuhlolwa, amasampuli anjalo kufanele afakwe phakathi noma ahlungwe ngaphambi kokusetshenziswa.

3.Amasampula ahlolwe angaba i-heparin, i-Sodium citrate noma i-EDTA anticoagulant plasma.

4.Ngokwezinqubo ezijwayelekile qoqa isampula.I-Serum noma isampula ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku ezingu-3 kanye nokugcinwa kwe-cryopreservation ngaphansi -15°C izinyanga ezingu-3.

5.Wonke amasampula agwema imijikelezo yokuncibilika kweqhwa.