IHame

Ibice by'igikoresho cyo kwipimisha bisizwe na antibody irwanya EV71 mu karere k'ibizamini hamwe na ihene irwanya urukwavu IgG antibody mu karere kayobora.Lable pad yashizwemo na fluorescence yanditseho antibody ya EV71 hamwe ninkwavu IgG mbere.Iyo ugerageza icyitegererezo cyiza, antigen ya EV71 murugero ikomatanya na fluorescence yanditseho antibody ya anti EV71, hanyuma igakora imvange yumubiri.Mubikorwa bya chromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere kizamini, yahujwe na antibody ya anti-EV71, ikora urwego rushya.

Niba ari bibi, icyitegererezo ntikirimo enterovirus 71 IgM antibody, kugirango ubudahangarwa bw'umubiri budashobora kubaho.Nta murongo utukura uzaba ahantu hagaragara (T).Ntakibazo niba Anterovirus 71 IgM antibody ibaho murugero cyangwa ntayibeho, ibisigisigi bya zahabu bisigaye byanditseho imbeba irwanya abantu IgM monoclonal antibody hamwe na antibody yihene irwanya imbeba IgG ikozweho ahantu hagenzurwa ubuziranenge (C).Hanyuma agglutinates itezimbere ibara mugace kagenzura ubuziranenge, kandi umurongo utukura uzagaragara muri (C).Umurongo utukura nigipimo kigaragara mugace kagenzura ubuziranenge (C) kugirango harebwe niba hari ingero zihagije kandi niba inzira ya chromatografiya ari ibisanzwe.Irakoreshwa kandi nkigipimo cyimbere cyimbere kuri reagent.

Uburyo bw'ikizamini:

1.Ingero zapimwe zishobora kuba amaraso yose, harimo amaraso y'amaraso cyangwa amaraso ya Periferiya.Amaraso yose ntashobora kubikwa nyuma yo gukusanya.Nkwiye gukoreshwa vuba nyuma yo gukusanya.

2.Urugero rwa serumu rwakusanyirijwe hamwe ukurikije tekiniki zisanzwe.Serumu idashyushye ntishobora gukoresha.Ntabwo byemewe gukoresha lipemic, turbid cyangwa serumu yanduye.Kugaragaza ibintu muri serumu.Kandi imvura izagira ingaruka kubisubizo byikizamini, ingero zigomba kuba zashyizwe hagati cyangwa zungururwa mbere yo gukoreshwa.

3.Ingero zapimwe zishobora kuba heparin, citrate ya Sodium cyangwa plasma anticoagulant ya EDTA.

4. Ukurikije tekinike isanzwe ikusanya icyitegererezo.Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 3 hamwe no kubika munsi -15 ° C mumezi 3.

5.Icyitegererezo cyose wirinde gukonjesha.