IHame

Igice gifite anti cal coating McAb mukarere ka test hamwe na ihene irwanya urukwavu IgG antibody mukarere kayobora, ifatirwa kuri chromatografi ya membrane hakiri kare.Lable pad isizwe na zahabu ya colloidal yanditseho anti cal McAb na zahabu ya colloidal yanditseho urukwavu IgG antibody mbere.Iyo ugerageza icyitegererezo cyiza, inyana yicyitegererezo yahujwe na zahabu ya colloidal yanditseho anti cal McAb, kandi ikora urwego rwumubiri, kuko yemerewe kwimuka kuruhande rwibizamini, uruganda rwitwa cal conjugate rufatwa na anti cal coating McAb kuri membrane no kumiterere. “Ant cal coating McAb-cal-colloidal zahabu yanditseho anti cal McAb”, itsinda ryibizamini byamabara ryagaragaye mukarere ka test.Imbaraga zamabara zifitanye isano neza nibirimo inyana.Icyitegererezo kibi ntigitanga umurongo wikizamini kubera kubura zahabu ya colloidal conjugate cal complex.Ntakibazo cal ihari murugero cyangwa ntayo, hariho umurongo utukura ugaragara mukarere kerekanwe hamwe nakarere kagenzura ubuziranenge, bifatwa nkibipimo ngenderwaho byimbere mu gihugu.

Uburyo bw'ikizamini:

1.Kuramo inkoni y'icyitegererezo, winjizwe mucyitegererezo cy'umwanda, hanyuma usubize inkoni y'icyitegererezo inyuma, uhindure neza kandi uzunguze neza, subiramo ibikorwa inshuro 3.Cyangwa ukoresheje inkoni y'icyitegererezo watoranije urugero rwa 50mg rw'icyitegererezo, hanyuma ugashyiramo umuyoboro w'icyitegererezo urimo imyunyu ngugu, hanyuma ugasunika neza.

2. Koresha imiyoboro ya pipette ikoreshwa ifata icyitegererezo cyoroshye cyumurwayi wimpiswi, hanyuma ongeramo ibitonyanga 3 (hafi 100uL) mumiyoboro ya fecal hanyuma uhindure neza, shyira kuruhande.

3.Kuramo ikarita yikizamini mu mufuka wa file, uyishyire kumeza urwego hanyuma ushireho akamenyetso.

4.Kura ingofero muri sample ya sample hanyuma ujugunye ibitonyanga bibiri byambere byongeweho icyitegererezo, ongeramo 4.ibitonyanga (hafi 100uL) ntagituba cyavanze cyicyitegererezo cya verticaly hanyuma buhoro buhoro mucyitegererezo cyikarita hamwe na disiketi yatanzwe, tangira igihe.

5.Ibisubizo bigomba gusomwa muminota 10-15, kandi ntibyemewe nyuma yiminota 15