KA'IDA

Tushen yana da maganin rigakafin ƙwayar cuta McAb akan yankin gwaji da goat anti-zomo IgG antibody akan yankin sarrafawa, wanda aka liƙa zuwa membrane chromatography a gaba.Label pad an lullube shi da zinari mai launi mai lakabin anti cal McAb da kuma zinare mai alamar zomo IgG antibody a gaba.Lokacin gwada ingantaccen samfurin, cal ɗin da ke cikin samfurin ya zo tare da zinare colloidal mai lakabin anti cal McAb, kuma ya samar da hadaddun rigakafi, yayin da aka ba shi izinin yin ƙaura tare da tsiri na gwaji, hadaddun cal conjugate ana kama shi da maganin antical McAb akan membrane da tsari. "Anti cal coating McAb-cal-colloidal zinariya mai lakabin anti cal McAb" hadaddun, wani launi na gwaji ya bayyana a yankin gwaji.Ƙarfin launi yana da alaƙa daidai da abun ciki na cal.Samfura mara kyau baya samar da band ɗin gwaji saboda rashin haɗin gwal na haɗin gwal na colloidal.Komai cal ya kasance a cikin samfur ko a'a, akwai jajayen ratsin da ke bayyana akan yankin tunani da yankin sarrafa inganci, wanda ake ɗaukarsa azaman ingantattun ma'auni na kasuwanci na ciki.

Tsarin Gwaji:

1.Za a fitar da sandar samfurin, a saka a cikin samfurin najasa, sannan a mayar da sandar samfurin baya, a dunƙule sosai kuma a girgiza sosai, maimaita aikin sau 3.Ko kuma amfani da sandar samfurin da aka zabo samfurin najasa kusan 50mg, sannan a saka a cikin bututun samfurin najasa mai ɗauke da samfurin dilution, sannan a dunƙule sosai.

2. Yi amfani da samfurin pipette da za a iya zubar da shi, ɗauki samfurin najasar ƙanƙara daga mai ciwon zawo, sannan a ƙara digo 3 (kimanin 100uL) a cikin bututun samfurin fecal sannan a girgiza sosai, a ajiye.

3. Ɗauki katin gwajin daga jakar takarda, sanya shi a kan teburin matakin kuma yi alama.

4.Cire hula daga tube samfurin kuma jefar da na farko biyu saukad da diluted samfurin, ƙara 4.drops (game da 100uL) babu kumfa diluted samfurin a tsaye da kuma sannu a hankali a cikin samfurin da kyau na katin tare da bayar dispette, fara lokaci.

5. Ya kamata a karanta sakamakon a cikin mintuna 10-15, kuma ba shi da inganci bayan mintuna 15.