UMGAQO

Umtya une-anti cal coating iMcAb kwindawo yovavanyo kunye nebhokhwe yokuchasa imivundla ye-IgG kwingingqi yolawulo, ebotshelelwa kwi-membrane chromatography kwangaphambili.Iphedi elayibhile iqatywe ngegolide ecolloidal ebhalwe anti cal McAb kunye negolide eyicolloidal ebhalwe i-antibody yomvundla we-IgG kwangaphambili.Xa uvavanya isampulu eqinisekileyo, i-cal kwisampulu yesampulu edibene negolide ye-colloidal ebhalwe anti cal McAb, kwaye yenza i-immune complex, njengoko ivunyelwe ukuba ifudukele ecaleni komtya wovavanyo, i-cal conjugate complex ibanjwa yi-anti cal coating McAb kwi-membrane kunye nefom. I-“anti cal coating iMcAb-cal-colloidal gold ebhalwe anti cal McAb” complex, ibhendi yovavanyo enemibala yavela kwindawo yovavanyo.Ukuqina kombala kuhambelana kakuhle nomxholo we-cal.Isampuli engalunganga ayivelisi ibhendi yovavanyo ngenxa yokungabikho kwe-colloidal gold conjugate cal complex.Kungakhathaliseki ukuba i-cal ikhona kwisampulu okanye ayikho, kukho umgca obomvu ovela kummandla wereferensi kunye nommandla wolawulo lomgangatho, othathwa njengomgangatho wemigangatho yeshishini langaphakathi.

Inkqubo yoVavanyo:

1.Thatha intonga yesampulu, uyifake kwisampulu yelindle, emva koko ubeke intonga yesampulu ngasemva, jija uqinise kakuhle, phinda intshukumo ka-3.Okanye usebenzisa intonga yesampulu ichola malunga ne-50mg yesampuli yelindle, kwaye ufake kwityhubhu yesampulu yelindle equlethe isampulu yedilution, kwaye ujije ngokuqinileyo.

2. Sebenzisa isampulu yepipette elahlwayo thatha isampuli yelindle elibhityileyo kwisigulana sorhudo, emva koko wongeze amathontsi ama-3 (malunga ne-100uL) kwityhubhu yesampulu ye-fecal uze uvuthulule kakuhle, ubeke ecaleni.

3.Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.

4.Susa i-cap kwi-tube yesampuli kwaye ulahle i-drops yokuqala ye-diluted samples, yongeza i-4.drops (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokucotha kwisampulu kakuhle yekhadi kunye ne-dispette enikeziweyo, qalisa ixesha.

5.Isiphumo kufuneka sifundwe kwimizuzu eyi-10-15, kwaye ayisebenzi emva kwemizuzu eli-15.