KUMU

Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka antibody anti EV71 ma ka ʻāina hoʻāʻo a me ke kao anti rabbit IgG antibody ma ka ʻāina hoʻomalu.Hoʻopili ʻia ka Lable pad e ka fluorescence i kapa ʻia i ka anti EV71 antibody a me ka rabbit IgG ma mua.I ka ho'āʻo ʻana i ka laʻana maikaʻi, hui pū ka antigen EV71 i ka laʻana me ka fluorescence i kapa ʻia ʻo antibody EV71 antibody, a hoʻokumu i ka hui kūlohelohe.Ma lalo o ka hana o ka chromatography, ke kahe paʻakikī i ke ala o ka pepa absorbent, i ka wā i hala ai ka paʻakikī i ka ʻāina hoʻāʻo, ua hui pū ʻia me ka anti EV71 coating antibody, hana hou i ka paʻakikī.

Inā maikaʻi ʻole, ʻaʻole i loaʻa i ka hāpana enterovirus 71 IgM antibody, no laila ʻaʻole hiki ke hoʻokumu ʻia ka paʻakikī immune.ʻAʻohe laina ʻulaʻula ma ka wahi ʻike (T).ʻAʻohe mea pili i ka Enterovirus 71 IgM antibody i loko o ka specimen a i ʻole, ʻo ke koena colloidal gold-labeled mouse anti-human IgM monoclonal antibody a me ke kao anti-mouse IgG antibody i uhi ʻia ma ka wahi hoʻomalu maikaʻi (C).A laila hoʻomohala nā agglutinates i ke kala ma ka ʻāpana hoʻomalu maikaʻi, a ʻike ʻia ka laina ʻulaʻula ma (C).ʻO ka laina ʻulaʻula ka maʻamau i hōʻike ʻia ma ka ʻāpana hoʻomalu maikaʻi (C) no ka hoʻoholo ʻana inā lawa nā laʻana a inā he maʻamau ke kaʻina chromatography.Hoʻohana ʻia ia ma ke ʻano he maʻamau mana kūloko no nā reagents.

Kaʻina hana hoʻāʻo:

1. ʻO nā mea hoʻohālike i hoʻāʻo ʻia he koko holoʻokoʻa, me ke koko venous a i ʻole ke koko Peripheral.ʻAʻole hiki ke mālama ʻia ke koko holoʻokoʻa ma hope o ka ʻohi ʻana.Pono wau e hoʻohana koke ma hope o ka ʻohi ʻana.

2.ʻOhiʻia nā hōʻailona Serum aseptically e like me nāʻenehana maʻamau.ʻAʻole hiki ke hoʻohana i ka serum wela-inactivated.ʻAʻole ʻōlelo ʻia e hoʻohana i ka lipemic, turbid a contaminated serum.ʻO nā mea ʻāpana i ka serum.A e hoʻopilikia ka ua i nā hopena hoʻāʻo, pono e centrifuged a kānana ʻia nā laʻana ma mua o ka hoʻohana ʻana.

3. ʻO nā mea hoʻohālike i ho'āʻoʻia he heparin, Sodium citrate a iʻole EDTA anticoagulant plasma.

4.According i nā hana maʻamau e hōʻiliʻili i ka hāpana.Hiki ke mālama ʻia ka serum a i ʻole plasma sample i loko o ka friji ma 2-8 ℃ no 3 mau lā a me ka cryopreservation ma lalo o -15°C no 3 mahina.

5.All laʻana e pale i nā pōʻaiapuni freeze-thaw.