Zida Zodziwira Matenda a Matenda apuloteni yogwira ntchito ya C-reactive kwambiri

(kuyesa kwa fluorescence immunochromatographic)

Kugwiritsa ntchito poyezetsa matenda m'thupi kokha

Chonde werengani bwino phukusili musanagwiritse ntchito ndipo tsatirani malangizo mosamala. Kudalirika kwa zotsatira za mayeso sikungatsimikizidwe ngati pali kusiyana kulikonse kuchokera ku malangizo omwe ali mu phukusili.

KUGWIRITSA NTCHITO KOFUNIKA

Chida Chodziwira Matenda cha mapuloteni okhudzidwa kwambiri a C-reactive (kuwunika kwa immunochromatographic) ndi njira yoyesera ya fluorescence immunochromatographic yodziwira kuchuluka kwa mapuloteni a C-reactive (CRP) mu seramu ya anthu / plasma / magazi athunthu. Ndi chizindikiro chosadziwika cha kutupa. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Mayesowa ndi a akatswiri azaumoyo okha.

CHIDULE

Puloteni ya C-reactive ndi puloteni yopangidwa ndi lymphokine yomwe imapangidwa ndi maselo a chiwindi ndi epithelial. Imapezeka mu seramu ya anthu, madzi a m'mitsempha ya ubongo, madzi am'mimba ndi m'mimba, ndi zina zotero, ndipo ndi gawo la chitetezo chamthupi chosakhala chapadera. Patatha maola 6-8 kuchokera pamene kachilombo ka bakiteriya kayamba, CRP inayamba kukwera, maola 24-48 inafika pachimake, ndipo mtengo wake unkatha kufika nthawi mazana ambiri kuposa wabwinobwino. Pambuyo pochotsa kachilomboka, CRP inatsika kwambiri ndikubwerera mwakale mkati mwa sabata imodzi. Komabe, CRP siikula kwambiri pankhani ya matenda opatsirana ndi kachilomboka, zomwe zimapangitsa kuti pakhale maziko odziwira matenda oyamba, ndipo ndi chida chodziwira matenda opatsirana ndi kachilomboka kapena bakiteriya.

MFUNDO YA NJIRAYI

Nembanemba ya chipangizo choyesera imakutidwa ndi anti-CRP antibody pamalo oyesera ndipo anti-IgG antibody ya mbuzi pamalo olamulira. Mapepala olembera amakutidwa ndi anti-CRP antibody yolembedwa ndi fluorescence ndi anti-IgG ya kalulu pasadakhale. Poyesa chitsanzo chabwino, anti-CRP mu chitsanzo imaphatikizana ndi anti-CRP antibody yolembedwa ndi fluorescence, ndikupanga chisakanizo cha chitetezo chamthupi. Pogwiritsa ntchito immunochromatography, kuyenda kwa zovuta kumayang'ana pepala loyamwa, pamene zovutazo zidadutsa pamalo oyesera, zimaphatikizana ndi anti-CRP coating antibody, zimapanga zovuta zatsopano. Mlingo wa CRP umagwirizanitsidwa bwino ndi chizindikiro cha fluorescence, ndipo kuchuluka kwa CRP mu chitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.