Ikhithi yokuxilonga yeI-hypersensitive C-reactive protein

(ukuhlolwa kwe-fluorescence immunochromatographic)

Ukusetshenziswa kwe-in vitro diagnostic kuphela

Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo.Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE

I-Diagnostic Kit ye-hypersensitive C-reactive protein (fluorescence immunochromatographic assay) iwukuhlola kwe-fluorescence immunochromatographic ukuze kutholwe inani le-C-reactive protein (CRP) ku-serum yomuntu / plasma/ Igazi lonke.Kuyinkomba engaqondile yokuvuvukala.Wonke amasampula amahle kufanele aqinisekiswe ngezinye izindlela.Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO

I-C-reactive protein iyiphrotheni yesigaba esibucayi ekhiqizwa ukugqugquzelwa kwe-lymphokine kwesibindi namaseli e-epithelial.Itholakala ku-serum yomuntu, uketshezi lwe-cerebrospinal, uketshezi lwe-pleural nolwesisu, njll., futhi iyingxenye yendlela yokuzivikela komzimba engaqondile.6-8h ngemva kokuvela kokutheleleka ngebhaktheriya, i-CRP yaqala ukwanda, 24-48h yafinyelela inani eliphakeme, futhi inani eliphakeme lingafinyelela izikhathi ezingamakhulu ezivamile.Ngemva kokuqedwa kokutheleleka, i-CRP yehla kakhulu futhi yabuyela kokujwayelekile phakathi nesonto elilodwa.Kodwa-ke, i-CRP ayinyuki kakhulu esimweni sokutheleleka ngegciwane, okunikeza isisekelo sokuhlonza izinhlobo zezifo ezithathelwanayo kusenesikhathi, futhi iyithuluzi lokuhlonza izifo ezibangelwa amagciwane noma amagciwane.

ISIMISO SENKQUBO

Ulwelwesi lwedivayisi yokuhlola lumbozwe ngamasosha omzimba e-CRP endaweni yokuhlola kanye ne-anti-rabbit IgG yamasosha omzimba endaweni yokulawula.Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi anti-CRP antibody kanye ne-rabbit IgG kusengaphambili.Lapho kuhlolwa isampula elihle, i-antigen ye-CRP kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti CRP antibody, bese yenza ingxube yokuzivikela komzimba.Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-CRP yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha.Izinga le-CRP lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukugxila kwe-CRP kusampula kungatholwa nge-fluorescence immunoassay assay.