NHEYO

Kamudzira kemudziyo wekuyedza kakaputirwa neRotavirus Group A antigen panzvimbo yekuyedza uye mbudzi anti rabbit IgG antibody panzvimbo yekudzora. Lable pad inoputirwa nefluorescence yakanyorwa kuti anti Rotavirus Group A uye tsuro IgG pachine nguva. Pakuyedza positive sample, RV iri mu sample inosanganiswa nefluorescence yakanyorwa kuti anti Rotavirus Group A, uye inoumba immune mixture. Pasi pekushanda kwe immunochromatography, complex inoyerera ichienda kune bepa rinonyudza. Kana complex yapfuura nzvimbo yekuyedza, yakabatana ne anti-Rotavirus Group A coating antibody, inoumba complex itsva. Kana iri negative, hapana Rotavirus Group A antigen mu sample, saka immune complexs hadzigone kuumbwa, hapazovi nemutsara mutsvuku munzvimbo yekuonekwa (T). Pasinei nekuti group A rotavirus iripo here mu sample, mbeva inonzi latex-labeled IgG inoiswa chromatograph kunzvimbo yekudzora mhando (C) uye inobatwa ne goat anti-mouse IgG antibody. Mutsetse mutsvuku uchaonekwa munzvimbo yekudzora mhando (C). Mutsetse mutsvuku ndiwo mwero unoonekwa munzvimbo yekudzora hunhu (C) wekutarisa kana paine mienzaniso yakakwana uye kana maitiro echromatography akajairwa. Unoshandiswawo semwero wekudzora mukati memareagents.

Maitiro Ekuyedza:

1. Varwere vane zviratidzo vanofanira kuunganidzwa. Zvinoenderana nemishumo, kubuda kwakanyanya kwerotavirus mutsvina yevarwere vane gastroenteritis kunoitika mazuva 3-5 mushure mekutanga kwechirwere uye mazuva 3-13 mushure mekutanga kwezviratidzo. Kana sampuro ikatorwa nguva refu mushure mekuita manyoka, huwandu hwemaantigen hungasakwana kuti pave nemhinduro yakanaka.

2. Mienzaniso yacho inofanira kuunganidzwa mumudziyo wakachena, wakaoma, usingapindi mvura usina sipo uye zvinochengetedza.

3. Kune varwere vasina manyoka, sampuro dzetsvina dzakaunganidzwa hadzifanirwe kunge dziri pasi pe 1-2 gramu. Kune varwere vane manyoka, kana tsvina yacho iri yemvura, ndapota torai kanenge 1-2 ml yetsvina. Kana tsvina yacho iine ropa rakawanda uye madzihwa, ndapota torai sampuro zvakare.

4. Zvinokurudzirwa kuti muedze sampuli idzi nekukasika mushure mekutorwa, zvikasadaro dzinofanira kutumirwa kurabhoritari mukati memaawa matanhatu uye kuchengetwa pa2-8°C. Kana sampuli dzisina kuongororwa mukati memaawa makumi manomwe nemaviri, dzinofanira kuchengetwa patembiricha iri pasi pe -15°C.

5. Shandisa tsvina itsva pakuedza, uye shandisa sampuro dzetsvina dzakasanganiswa nemvura yakasanganiswa nemvura yakasanganiswa nemvura yakasanganiswa nemvura yakasanganiswa nemvura yakasanganiswa nemvura.