Kiti yekuongorora chirwere chepfungwamapuroteni eC-reactive anonyanya kunzwa zvakanyanya

(kuyedza kwe fluorescence immunochromatographic)

Kushandiswa kwekuongorora in vitro chete

Ndapota verengai zviri mupakeji iyi nokungwarira musati mashandisa uye teverai mirairo yacho nemazvo. Kuvimbika kwemhedzisiro yekuyedza hakugone kuvimbiswa kana paine chero kutsauka kubva pamirairo iri mupakeji iyi.

KUSHANDISA KWAKATARISIRWA

Diagnostic Kit yekuongorora mapuroteni eC-reactive ane hypersensitive (fluorescence immunochromatographic assay) inzira yekuongorora mapuroteni eC-reactive (CRP) muropa revanhu /plasma / Ropa rese. Ichi chiratidzo chisina kujeka chekuzvimba. Sampuro yese yakanaka inofanira kusimbiswa nedzimwe nzira. Bvunzo iyi yakagadzirirwa kushandiswa nenyanzvi dzezvehutano chete.

PFUPISO

Mapuroteni eC-reactive ipuroteni yechikamu cheacute inogadzirwa nekukurudzirwa kwemasero echiropa neepithelial muropa. Inowanikwa muropa revanhu, muropa remumusana, mumvura yemudumbu, nezvimwewo, uye chikamu chemuviri wemuviri usingashande zvakanaka. Maawa 6-8 mushure mekuonekwa kwehutachiona hwebhakitiriya, CRP yakatanga kuwedzera, maawa 24-48 yakasvika pakakwirira, uye huwandu hwepamusoro hwaigona kusvika mazana enguva dzakajairika. Mushure mekubviswa kwehutachiona, CRP yakaderera zvakanyanya ndokudzokera pakare mukati mevhiki imwe chete. Zvisinei, CRP haiwedzere zvakanyanya kana paine hutachiona hwehutachiona, izvo zvinopa hwaro hwekuzivikanwa kwezvirwere zvekutanga, uye chishandiso chekuona hutachiona hwehutachiona kana hwebhakitiriya.

NZVIMBO YEMASHURE

Kamudzira kemudziyo wekuyedza kakaputirwa neanti CRP antibody panzvimbo yekuyedza uye anti mbudzana IgG antibody panzvimbo yekudzora. Mapepa eLable pad anoputirwa ne fluorescence yakanyorwa kuti anti CRP antibody uye tsuro IgG pachine nguva. Pakuyedza positive sample, CRP antigen iri mu sample inosangana ne fluorescence yakanyorwa kuti anti CRP antibody, uye inoumba immune mixture. Pasi pekushanda kwe immunochromatography, complex flow inoenda kune absorbent paper, kana complex yapfuura nzvimbo yekuyedza, yakabatana ne anti CRP coating antibody, inoumba complex itsva. CRP level inoenderana zvakanaka ne fluorescence signal, uye kuwanda kweCRP mu sample kunogona kuonekwa ne fluorescence immunoassay assay.