Qalabka Baadhista ee loogu talagalayBorotiinka C-reactive-ka ee xasaasiga ah

(tijaabinta immunochromatographic fluorescence)

Kaliya loogu talagalay isticmaalka ogaanshaha in vitro

Fadlan si taxaddar leh u akhri xirmadan geli ka hor intaadan isticmaalin oo si adag u raac tilmaamaha. Kalsoonida natiijooyinka tijaabada lama dammaanad qaadi karo haddii ay jiraan wax ka leexasho ah tilmaamaha ku jira xirmadan geli.

ISTICMAALKA LOOGU TALAGALAY

Qalabka Baadhista ee borotiinka C-reactive-ka ee xasaasiga ah (tijaabada fluorescence immunochromatographic) waa tijaabo fluorescence immunochromatographic ah oo lagu ogaanayo tirada borotiinka C-reactive (CRP) ee ku jira serumka aadanaha /plasma / dhiigga oo dhan. Waa tilmaame aan gaar ahayn oo barar ah. Dhammaan muunadaha togan waa in lagu xaqiijiyaa habab kale. Baaritaankan waxaa loogu talagalay isticmaalka xirfadleyda daryeelka caafimaadka oo keliya.

SOO KOOBID

Borotiinka C-reactive waa borotiin marxalad degdeg ah oo ay soo saarto kicinta lymphokine ee unugyada beerka iyo epithelial. Waxay ku jirtaa seeramka aadanaha, dareeraha maskaxda, dareeraha sambabada iyo caloosha, iwm., waana qayb ka mid ah habka difaaca jirka ee aan gaarka ahayn. 6-8 saacadood ka dib dhacdada caabuqa bakteeriyada, CRP wuxuu bilaabay inuu kordho, 24-48 saacadood wuxuu gaaray meesha ugu sarreysa, qiimaha ugu sarreeyana wuxuu gaari karaa boqolaal jeer oo caadi ah. Ka dib markii la tirtiray caabuqa, CRP si xoog leh ayuu hoos ugu dhacay wuxuuna ku soo laabtay caadi hal toddobaad gudahood. Si kastaba ha ahaatee, CRP si weyn uma kordho marka uu dhaco caabuqa fayraska, kaas oo saldhig u ah aqoonsiga noocyada caabuqyada hore ee cudurrada, waana qalab lagu aqoonsado caabuqyada fayraska ama bakteeriyada.

MABAADI'DA HABKA

Xuubka qalabka tijaabada waxaa lagu dahaadhay antibody-ka ka hortagga CRP ee gobolka tijaabada iyo antibody-ka ka hortagga bakaylaha riyaha ee gobolka xakamaynta. Suunka label-ka waxaa lagu dahaadhay antibody-ka ka hortagga CRP ee calaamadaysan fluorescence iyo IgG-ga bakaylaha kahor. Marka la tijaabinayo muunad togan, antigen-ka CRP ee muunadda ku jira ayaa isku daraya antibody-ka ka hortagga CRP ee calaamadaysan fluorescence, waxayna sameeyaan isku darka difaaca jirka. Iyada oo la raacayo ficilka immunochromatography, socodka isku dhafan ee jihada warqadda nuugta, marka isku-dhafka uu dhaafo gobolka tijaabada, oo lagu daro antibody-ka dahaarka CRP ee ka hortagga, wuxuu sameeyaa isku-dhafan cusub. Heerka CRP wuxuu si togan ula xiriiraa calaamadda fluorescence, fiirsashada CRP ee muunadda waxaa lagu ogaan karaa baaritaanka immunoassay fluorescence.