Ikhithi yokuxilonga yeiprotheyini esebenzayo ye-C ebuthathaka kakhulu

(uvavanyo lwe-fluorescence immunochromatographic)

Ukusetyenziswa kokuxilongwa kwi-vitro kuphela

Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO

Isixhobo sokuxilonga seprotheyini ye-C-reactive ebuthathaka kakhulu (uvavanyo lwe-immunochromatographic lwe-fluorescence) luvavanyo lwe-immunochromatographic lwe-fluorescence lokufumanisa ubungakanani beprotheyini ye-C-reactive (CRP) kwi-serum yomntu /plasma / igazi elipheleleyo. Luphawu olungangqalanga lokudumba. Zonke iisampulu ezilungileyo kufuneka ziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zonyango kuphela.

ISISHWANKATHELO

Iprotheyini ye-C-reactive yiprotheyini yesigaba esibukhali eveliswa kukukhuthazwa kwe-lymphokine kwiiseli zesibindi kunye ne-epithelial. Ifumaneka kwi-serum yomntu, kwi-cerebrospinal fluid, kwi-pleural nakwisisu, njl.njl., kwaye iyinxalenye yendlela yokuzikhusela yomzimba engeyiyo ethile. Kwiiyure ezi-6-8 emva kokuba usulelo lwebhaktiriya luqalile, i-CRP yaqala ukwanda, iiyure ezingama-24-48 zafikelela kwincopho, kwaye ixabiso eliphezulu linokufikelela kumakhulu amaxesha aqhelekileyo. Emva kokupheliswa kosulelo, i-CRP yehla kakhulu yaza yabuyela kwisiqhelo kwiveki enye. Nangona kunjalo, i-CRP ayinyuki kakhulu kwimeko yosulelo lwentsholongwane, nto leyo enika isiseko sokuchongwa kweentlobo zosulelo lwasekuqaleni lwezifo, kwaye sisixhobo sokuchonga usulelo lwentsholongwane okanye lwebhaktiriya.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-CRP antibody kwindawo yovavanyo kunye ne-anti-rabbit IgG antibody yebhokhwe kwindawo yokulawula. I-Lable pad igqunywe nge-fluorescence ebhalwe nge-anti-CRP antibody kunye ne-rabbit IgG kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-antigen ye-CRP kwisampuli idibana ne-anti-CRP antibody ebhalwe nge-fluorescence, kwaye yenze umxube womzimba. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlule kwindawo yovavanyo, idibene ne-anti-CRP coating antibody, yenza i-complex entsha. Inqanaba le-CRP lihambelana kakuhle nesignali ye-fluorescence, kwaye uxinzelelo lwe-CRP kwisampuli lunokufunyanwa ngovavanyo lwe-fluorescence immunoassay.