UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-microalbumin antigen kwindawo yovavanyo kunye ne-antibody yebhokhwe ye-IgG antibody kwindawo yokulawula. I-lable pad igqunywe yi-fluorescence ebhalwe nge-microalbumin kunye ne-rabbit IgG kwangaphambili. Ukuba akukho albumin kumchamo, i-antibody ye-colloidal gold-labeled anti-Alb-labeled monoclonal ephepheni le-colloidal gold iya kuhamba kwi-membrane kunye nomchamo ukuya kumgca wokufumanisa, kwaye idibane ne-antigen ye-Alb-coated enomgca obonakalayo. Kwaye umbala womgca umnyama kunombala womgca kwindawo yokulawula (C), esi sisiphumo esibi. Ukuba umchamo uqulethe i-albumin, baya kukhuphisana ne-antigen ye-Alb-coated kwi-membrane ukuze banamathele kwiindawo ze-antibody ezilinganiselweyo kwi-antibody ye-colloidal gold-labeled anti-Alb-labeled monoclonal. Njengoko inani le-albumin kumchamo lisanda, uvavanyo

Umbala womgca uya kuba mhlophe ngakumbi nangakumbi. Umxholo we-albumin kumchamo unokubonwa ngokwemilinganiselo ngokuthelekisa indawo yokufumanisa (T) nendawo yokulawula (C). Indawo yokulawula umgangatho (C) kunye nendawo yokujonga (R) kwikhithi ziya kuhlala zivela ngexesha lovavanyo, kwaye azinanto yakwenza nokuba khona kwe-albumin yomchamo. Indawo yokulawula (C) kunye nomgca wendawo yokujonga (R) zingasetyenziswa njengesalathisi sangaphakathi solawulo lomgangatho kwikhithi.

Inkqubo yoVavanyo:

Nceda ufunde incwadi yemiyalelo yokusebenza kwesixhobo kunye nesiqulatho sephakheji ngaphambi kokuvavanya. Nyibilikisa iisampulu zibe kubushushu begumbi ngaphambi kokusebenzisa.

1. Khupha ikhadi lovavanyo kwingxowa yefoyile. Libeke tyaba phezu komphezulu othe tyaba uze uliphawule.

2.Thatha isampuli yomchamo ngepipette elahlwayo, ulahle amathontsi amabini okuqala esampuli yomchamo. Yongeza amathontsi amathathu (malunga ne-100uL) omchamo ongenamaqamza embindini womngxuma wesampuli yekhadi lovavanyo ngokuthe nkqo uze uqale ixesha.

3. Funda iziphumo emva kwemizuzu eli-10-15. Ayisebenzi ukuba ingaphezulu kwemizuzu eli-15.