Ikhithi Yokuxilonga(I-1965)Igolide le-Colloidal）i-Follicle-stimulating Hormone
Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela
Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Le khithi isetshenziselwa ukuthola amazinga e-follicle-stimulating hormone (FSH) kumasampula omchamo. Ifanele ukusiza ekunqumeni ukubonakala kokunqamuka kokuya esikhathini kwabesifazane.

USAYIZI WEPAKETHE

Ikhithi eli-1 /ibhokisi, amakhithi ayi-10 /ibhokisi, amakhithi angama-25, /ibhokisi, amakhithi angama-50 /ibhokisi.

ISIFINYEZO

I-FSH iyi-hormone ye-glycoprotein ekhishwa yi-pituitary gland, ingangena egazini nasemchameni ngokujikeleza kwegazi. Kwabesilisa, i-FSH ikhuthaza ukuvuthwa kwe-testicular seminiferous tubule kanye nokukhiqizwa kwesidoda, kwabesifazane, i-FSH ikhuthaza ukukhula kwe-follicular kanye nokuvuthwa, futhi isebenzisana ne-LH kuma-follicle avuthiwe akhipha i-estrogen kanye ne-ovulation, ehilelekile ekwakhekeni kokuya esikhathini okuvamile[1]. I-FSH igcina izinga eliyisisekelo elizinzile njalo kubantu abavamile, cishe i-5-20mIU/mL. Ukunqamuka kokuya esikhathini kwabesifazane kuvame ukwenzeka phakathi kweminyaka engu-49 no-54, futhi kuhlala isilinganiso seminyaka emine kuya kwemihlanu. Phakathi nalesi sikhathi, ngenxa yokuncipha kwama-ovari, i-follicular atresia kanye nokuwohloka, ukukhishwa kwe-estrogen kwehle kakhulu, inani elikhulu lokukhishwa kwe-pituitary gonadotropin okushukumisayo, ikakhulukazi amazinga e-FSH azokhuphuka kakhulu, ngokuvamile angama-40-200mIU/ml, futhi agcine izinga esikhathini eside kakhulu.^[2]. Le khithi isekelwe kubuchwepheshe bokuhlaziya i-colloidal gold immune chromatography ukuthola i-antigen ye-FSH esezingeni eliphezulu kumasampula omchamo womuntu, okunganikeza umphumela zingakapheli imizuzu eyi-15.

INQUBO YOKUHLOLA
1. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni elilinganayo bese ulimaka.
2. Lahla isampula yokuqala yamaconsi amabili, engeza amaconsi amathathu (cishe i-100μL) ngaphandle kwesampula yebhamuza ngokuqondile bese kancane kancane uyifaka emthonjeni wesampula wekhadi nge-dispette enikeziwe, qala isikhathi.
3. Umphumela kufanele ufundwe zingakapheli imizuzu eyi-10-15, futhi awusebenzi ngemva kwemizuzu eyi-15.