Ikhithi Yokuxilonga yeiphrotheni esabela kakhulu ye-C

(ukuhlolwa kwe-fluorescence immunochromatographic)

Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela

Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Ikhithi Yokuxilonga yeprotheyini ye-C-reactive ezwela kakhulu (i-fluorescence immunochromatographic assay) iyi-fluorescence immunochromatographic assay yokuthola inani leprotheyini ye-C-reactive (CRP) ku-serum yomuntu /plasma / igazi lonke. Kuyinkomba engacacile yokuvuvukala. Wonke amasampula amahle kumele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.

ISIFINYEZO

Iphrotheni ye-C-reactive iyiphrotheni yesigaba esiphuthumayo ekhiqizwa ukukhuthazwa kwe-lymphokine kwamaseli esibindi kanye ne-epithelial. Itholakala ku-serum yomuntu, uketshezi lwe-cerebrospinal, uketshezi lwe-pleural kanye nolwesisu, njll., futhi iyingxenye yendlela yokuzivikela yomzimba engeyona ethile. Ngemva kwamahora angu-6-8 ukutheleleka ngamagciwane, i-CRP yaqala ukwanda, amahora angu-24-48 yafinyelela esicongweni, futhi inani eliphakeme lingafinyelela izikhathi ezingamakhulu zokujwayelekile. Ngemva kokususwa kokutheleleka, i-CRP yehla kakhulu futhi yabuyela esicongweni phakathi nesonto elilodwa. Kodwa-ke, i-CRP ayikhuphuki kakhulu uma kwenzeka ukutheleleka ngamagciwane, okunikeza isisekelo sokuhlonza izinhlobo zezifo zokutheleleka kwasekuqaleni, futhi iyithuluzi lokuhlonza ukutheleleka ngamagciwane noma ngamagciwane.

ISIMISO SENQUBO

I-membrane yedivayisi yokuhlola imbozwe nge-anti-CRP antibody endaweni yokuhlola kanye ne-anti-IgG antibody yembuzi endaweni yokulawula. I-lable pad imbozwe nge-anti-CRP antibody ebhalwe nge-fluorescence kanye ne-IgG yenogwaja kusengaphambili. Lapho kuhlolwa isampula enhle, i-antigen ye-CRP kusampula ihlangana ne-anti-CRP antibody ebhalwe nge-fluorescence, bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ohlangothini lwephepha elimuncayo, lapho i-complex idlula esifundeni sokuhlola, ihlanganiswe ne-anti-CRP coating antibody, yakha i-complex entsha. Izinga le-CRP lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukuhlushwa kwe-CRP kusampula kungatholakala nge-fluorescence immunoassay assay.