Kit ɗin Bincike don Jimlar Triiodothyronine(Fluorescence immunochromatographic assay)
Don bincikar in vitro amfani kawai
Da fatan za a karanta wannan fakitin a hankali kafin amfani kuma a bi umarnin sosai.Ba za a iya tabbatar da amincin sakamakon kima ba idan akwai wasu sabani daga umarnin a cikin wannan fakitin.

AMFANI DA NUFIN
Kit ɗin Diagnostic don Total Triiodothyronine (fluorescence immunochromatographic assay) ne mai haske immunochromatographic kididdigar don ƙididdige yawan gano jimlar Triiodothyronine (TT3) a cikin jini ko jini na ɗan adam, wanda aka fi amfani dashi don kimanta aikin thyroid.It ne mai karin bincike reagent.All tabbatacce samfurin reagent. dole ne a tabbatar da wasu hanyoyin.Anyi nufin wannan gwajin don amfanin ƙwararrun kiwon lafiya kawai.

TAKAITACCEN
Triiodothyronine (T3) nauyin kwayoyin 651D.Shi ne babban nau'i mai aiki na hormone thyroid.Jimlar T3 (Total T3, TT3) a cikin maganin an raba shi zuwa nau'ikan ɗauri da kyauta.99.5 % na TT3 yana ɗaure da maganin Thyroxine Binding Proteins (TBP), kuma T3 (Free T3) kyauta yana lissafin 0.2 zuwa 0.4%.T4 da T3 suna shiga cikin kiyayewa da daidaita tsarin aikin jiki na jiki. Ana amfani da ma'auni na TT3 don kimanta yanayin aikin thyroid da ganewar cututtuka.Clinical TT3 shine alamar abin dogara don ganewar asali da kuma ingancin lura da hyperthyroidism da hypothyroidism. Ƙaddamar da T3 ya fi mahimmanci don ganewar cutar hyperthyroidism fiye da T4.

KA'IDAR HANYA

An lulluɓe membrane na na'urar gwajin tare da haɗin BSA da T3 akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa.Alamar alamar ana lullube ta da alamar fluorescence anti T3 antibody da zomo IgG a gaba.Lokacin gwada samfurin, TT3 a cikin samfurin haɗa tare da kyalli alama anti-T3 antibody, da kuma samar da rigakafi cakuda.A karkashin aikin na immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun ya wuce gwajin yankin, The free kyalli alama za a hade tare da T3 a kan membrane.The maida hankali na TT3 ne korau dangantaka ga haske sigina, da kuma Ana iya gano maida hankali na TT3 a cikin samfurin ta hanyar gwajin immunoassay fluorescence.

REAgents DA KAYAN DA AKA BAYAR

Abubuwan kunshin 25T:
.Katin gwajin daidaiku da foil ɗin da aka shayar da shi tare da 25T na busa
.Mafita 25T
.B Magani 1
. Kunshin saka 1

KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
Samfurin tarin akwati, mai ƙidayar lokaci

MISALI TATTAUNAWA DA AJIYA
1. Samfuran da aka gwada na iya zama jini, heparin anticoagulant plasma ko EDTA anticoagulant plasma.
2.According ga daidaitattun dabarun tattara samfurin.Za'a iya adana ruwan magani ko samfurin plasma a cikin firiji a 2-8 ℃ na tsawon kwanaki 7 kuma ana kiyaye cryopreservation ƙasa -15 ° C na watanni 6.
3.All samfurin guje wa daskare-narke hawan keke.

HANYAR ASSAY
Hanyar gwaji na kayan aiki duba jagorar immunoanalyzer.Hanyar gwajin reagent shine kamar haka
1.Lay a gefe duk reagents da samfurori zuwa dakin zafin jiki.
2.Bude Portable Immune Analyzer (WIZ-A101), shigar da kalmar sirri shiga asusu bisa ga tsarin aiki na kayan aiki, da kuma shigar da ganowa dubawa.
3.Scan da lambar haƙori don tabbatar da gwajin abu.
4.Dauki katin gwaji daga jakar foil.
5.Saka katin gwaji a cikin ramin katin, duba lambar QR, kuma ƙayyade abin gwajin.
6.Add 30μL serum ko plasma samfurin zuwa A bayani, da kuma Mix da kyau.
7.Add 20μL B bayani zuwa ga cakuda na sama, da kuma Mix da kyau.
8.Bar cakuda don minti 20.
9.Add 80μL cakuda zuwa samfurin rijiyar katin.
10. Danna maɓallin "misali gwajin", bayan minti 10, kayan aiki za su gano katin gwajin ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aiki, kuma rikodin / buga sakamakon gwajin.
11. Koma zuwa ga umarnin Portable Immune Analyzer (WIZ-A101).

DABI'UN DA AKE SARANSU

TT3 na al'ada: 0.5-2.5ng/ml
Ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kafa nasa kewayon al'ada wanda ke wakiltar yawan majinyacinsa.

SAKAMAKON JARRABAWA DA FASSARA
.Bayanan da ke sama shine tazara tazara da aka kafa don gano bayanan wannan kit ɗin, kuma ana ba da shawarar cewa kowane ɗakin gwaje-gwaje ya kamata ya kafa tazara tazara don mahimmancin asibiti da ya dace na yawan jama'a a wannan yanki.
.Tsarin TT3 ya fi girma fiye da ma'anar tunani, kuma ya kamata a cire sauye-sauye na ilimin lissafin jiki ko amsawar damuwa.Hakika maras kyau, ya kamata ya hada da ganewar asali na asibiti.
.Sakamakon wannan hanyar yana aiki ne kawai ga kewayon tunani da aka kafa ta wannan hanyar, kuma sakamakon ba ya daidaita kai tsaye da sauran hanyoyin.
.Wasu dalilai kuma na iya haifar da kurakurai a cikin sakamakon ganowa, gami da dalilai na fasaha, kurakuran aiki da sauran abubuwan samfuri.

AJIYA DA KWANTA
1.The kit ne 18 watanni shiryayye-rai daga ranar da aka yi.Ajiye kayan da ba a yi amfani da su ba a 2-30 ° C.KAR KA DANKE.Kar a yi amfani da bayan ranar karewa.
2.Kada ku buɗe jakar da aka rufe har sai kun shirya don yin gwaji, kuma ana ba da shawarar yin amfani da gwajin amfani ɗaya a ƙarƙashin yanayin da ake buƙata (zazzabi 2-35 ℃, zafi 40-90%) a cikin 60 mins da sauri. kamar yadda zai yiwu.
3.Sample diluent ana amfani da shi nan da nan bayan an bude shi.

GARGADI DA TSIRA
.Kit ɗin yakamata a rufe shi kuma a kiyaye shi daga danshi.
.Duk samfurori masu inganci za a inganta su ta wasu hanyoyin.
.Duk samfuran za a kula da su azaman masu gurɓata yanayi.
.KADA KA yi amfani da reagent da ya ƙare.
.KADA KA musanya reagents tsakanin kits tare da kuri'a daban-daban.
.KADA KA sake amfani da katunan gwaji da duk wani kayan haɗi da za'a iya zubarwa.
.Misoperation, wuce kima ko kadan samfurin iya haifar da sabawa sakamakon.

LIMITATION
Kamar yadda yake tare da kowane gwajin amfani da ƙwayoyin rigakafi na linzamin kwamfuta, akwai yuwuwar kutsawa daga ƙwayoyin rigakafin linzamin kwamfuta na ɗan adam (HAMA) a cikin samfurin.Samfura daga marasa lafiya waɗanda suka karɓi shirye-shiryen rigakafin ƙwayoyin cuta na monoclonal don ganewar asali ko jiyya na iya ƙunsar HAMA.Irin waɗannan samfurori na iya haifar da sakamako mara kyau na ƙarya ko ƙarya.
.Wannan sakamakon gwajin shine kawai don tunani na asibiti, bai kamata ya zama tushen kawai don ganewar asibiti da magani ba, kula da lafiyar marasa lafiya ya kamata a yi la'akari da shi sosai tare da bayyanar cututtuka, tarihin likita, sauran gwaje-gwaje na dakin gwaje-gwaje, amsawar magani, cututtukan cututtuka da sauran bayanai. .
.Wannan reagent ana amfani dashi kawai don gwajin jini da jini.Maiyuwa baya samun ingantaccen sakamako idan aka yi amfani da shi don wasu samfura kamar yau da fitsari da sauransu.

HALAYEN YI

REFERENCES
1.Hansen JH, et al.HAMA Tsangwama tare da Murine Monoclonal Antibody-Based Immunoassays[J].J na Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Yanayin Heterophilic Antibodies da Matsayin Tsangwama na Immunoassay[J].J na Clin Immunoassay,1992,15:108-114.

Maɓalli ga alamomin da aka yi amfani da su:

	In Vitro Diagnostic Medical Na'urar
	Mai ƙira
	Adana a 2-30 ℃
	Ranar Karewa
	Kada a sake amfani
	HANKALI
	Tuntuɓi Umarnin Don Amfani

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