MFUNDO

Chipolopolo cha chipangizo choyesera chimakutidwa ndi antibody ya Cpn-IgM pamalo oyesera ndipo antibody ya mbuzi ya IgG yotsutsana ndi kalulu pamalo olamulira. Mapepala olembedwa amakutidwa ndi antibody ya fluorescence yolembedwa kuti anti Cpn-IgM ndi antibody ya kalulu IgG pasadakhale. Poyesa chitsanzo chabwino, antigen ya Cpn-IgM mu chitsanzocho imaphatikizana ndi antibody ya fluorescence yolembedwa kuti anti Cpn-IgM, ndikupanga chisakanizo cha chitetezo chamthupi. Pansi pa ntchito ya immunochromatography, zovuta zimapita molunjika ku pepala loyamwa. Pamene zovuta zimadutsa pamalo oyesera, zimaphatikizana ndi antibody ya anti Cpn-IgM, zimapanga zovuta zatsopano. Mzere wofiira ndi muyezo womwe umawonekera m'dera lowongolera khalidwe (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yachibadwa. Imagwiritsidwanso ntchito ngati muyezo wamkati wa ma reagents.

Njira Yoyesera

Njira yoyesera ya WIZ-A202 ndi Portable Immune analyzer WIZ-A101 zimatanthauza malangizo a malangizowo. Njira yoyesera yowonera ndi iyi:

1. Tulutsani khadi loyesera m'thumba la foil, liyikeni patebulo lolingana ndipo liyikeni chizindikiro.
2. Onjezani 10μl ya seramu kapena plasma kapena 20ul ya magazi athunthu kuti muyese chitsime cha khadi lomwe lili ndi dispette yoperekedwa, kenako onjezerani 100μl (pafupifupi madontho 2-3) a chitsanzo choyeretsera; nthawi yoyambira.
3. Yembekezerani kwa mphindi zosachepera 10-15 kenako muwerenge zotsatira zake, zotsatira zake zimakhala zosavomerezeka patatha mphindi 15.