Agakoresho ko gusuzuma indwara ka Triiodothyronine yose(isuzuma rya fluorescence immunochromatographic assay)
Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa
Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE
Ikizamini cyo gusuzuma Triiodothyronine yose (ikizamini cya fluorescence immunochromatographic assay) ni ikizamini cya fluorescence immunochromatographic assay cyo gupima ingano ya Triiodothyronine yose (TT3) mu maraso y'umuntu cyangwa mu maraso, gikoreshwa cyane cyane mu gusuzuma imikorere ya tiroyide. Ni reagent y'inyongera yo gusuzuma. Ikizamini cyose cyagaragaye ko gifite ibimenyetso kigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

INSHAMAKE
Uburemere bwa molekile ya Triiodothyronine (T3) 651D. Ni bwo bwoko bw'ingenzi bw'imisemburo ya tiroyide. T3 yose (T3 yose, TT3) mu maraso igabanyijemo ubwoko bwo gufatana n'ubw'abantu. 99.5% bya TT3 bifatana na poroteyine zo gufatana za Thyroxine (TBP) mu maraso, naho T3 y'abantu (T3 y'abantu) ingana na 0.2 kugeza kuri 0.4%. T4 na T3 bigira uruhare mu kubungabunga no kugenzura imikorere y'umubiri. Ibipimo bya TT3 bikoreshwa mu gusuzuma imikorere ya tiroyide no gusuzuma indwara. TT3 yo kwa muganga ni ikimenyetso cyizewe cyo gusuzuma no kureba neza indwara ya hyperthyroidism na hypothyroidism. Kumenya T3 ni ingenzi cyane mu gusuzuma hyperthyroidism kurusha T4.

IHAME RY'UBURYO BWAYO

Agace k'igikoresho cyo gupima gatwikiriwe n'agace ka BSA na T3 ku gace k'igeragezwa n'agace ka anti-inkwavu ka IgG gatwikiriwe n'ihene ku gace k'igenzura. Agapapuro k'ikarita gatwikiriwe n'agace ka anti-inkwavu ka T3 gatwikiriwe n'agace ka IgG mbere y'igihe. Mu gihe cyo gupima icyitegererezo, TT3 mu gipimo ihuzwa n'agace ka anti-inkwavu ka T3 gatwikiriwe n'urumuri, maze igakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'imikorere ya immunochromatography, urujya n'uruza rw'urumuri mu cyerekezo cy'impapuro zifata, iyo agace kanyuze mu gace k'igerageza, ikimenyetso cy'urumuri cyigenga kizahuzwa na T3 ku gakanu. Ingano ya TT3 ni isano iri hagati y'ikimenyetso cy'urumuri, kandi ingano ya TT3 mu gipimo ishobora kumenyekana hakoreshejwe isuzuma ry'ubudahangarwa bw'urumuri.

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T:
Ikarita yo gupima ku giti cyayo ipfunyitse mu gapfunyika gafite agakoresho ko gusukura 25T
.Igisubizo 25T
Igisubizo cya .B 1
.Ipaki ishyirwamo 1

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA
Agasanduku ko gukusanya ingero, igihe

ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
1. Ingero zapimwe zishobora kuba serum, heparin anticoagulant plasma cyangwa EDTA anticoagulant plasma.
2. Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 7 no kubikwa munsi ya -15°C mu gihe cy'amezi 6.
3. Ingero zose zirinda gukonjesha no gushonga.

UBURYO BWO GUKORA IKIGANIRO
Uburyo bwo gupima igikoresho reba igitabo cy'amabwiriza agenga ikoreshwa ry'imyunyungugu. Uburyo bwo gupima reagent ni ubu bukurikira
1. Shyira ku ruhande ibikoresho byose bikoreshwa mu gupima no mu gupima ingero ku bushyuhe bw'icyumba.
2. Fungura Portable Immune Analyzer (WIZ-A101), andika ijambo ry'ibanga ryinjira muri konti ukurikije uburyo bw'imikorere y'igikoresho, hanyuma winjize aho ureba.
3.Skena kode y'isuzuma kugira ngo wemeze ikintu cy'ikizamini.
4. Kura ikarita yo gupima mu gafuka k'ifishi.
5. Shyiramo ikarita y'ikizamini mu mwanya w'ikarita, skana kode ya QR, hanyuma umenye ikintu cy'ikizamini.
6. Shyiramo serum cyangwa plasma ya 30μL mu gisubizo cya A, hanyuma uvange neza.
7. Shyiramo umuti wa 20μL B kuri urwo ruvange rwavuzwe haruguru, hanyuma uvange neza.
8. Reka uruvange mu gihe cy'iminota 20.
9. Shyiramo uruvange rwa 80μL kugira ngo ufate urugero rw'ikarita.
10. Kanda kuri buto "ikizamini gisanzwe", nyuma y'iminota 10, igikoresho kizahita kibona ikarita y'ikizamini, gishobora gusoma ibisubizo bivuye kuri ecran y'igikoresho, no kwandika/gucapa ibisubizo by'ikizamini.
11. Reba amabwiriza ya Portable Immune Analyzer (WIZ-A101).

INDANGAGACIRO ZITEGANYIJWE

TT3 isanzwe: 0.5-2.5ng/mL
Birasabwa ko buri laboratwari ishyiraho urwego rwayo rusanzwe rugaragaza umubare w’abarwayi bayo.

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO
.Amakuru yavuzwe haruguru ni igihe cyo gusuzuma amakuru y’iyi kit, kandi birasabwa ko buri laboratwari igomba gushyiraho igihe cyo gusuzuma amakuru ajyanye n’akamaro k’ubuvuzi ku baturage bo muri aka gace.
.Ingano ya TT3 iri hejuru y’urugero rw’ifatizo, kandi impinduka z’umubiri cyangwa igisubizo cy’imihangayiko ntizigomba kurebwa. Mu by’ukuri, bigomba guhuza ibimenyetso by’indwara.
Ibisubizo by'ubu buryo bireba gusa urwego rw'ifatizo rwashyizweho n'ubu buryo, kandi ibisubizo ntibigereranywa neza n'ubundi buryo.
Izindi mpamvu zishobora gutera amakosa mu bisubizo byo kuvumbura, harimo impamvu za tekiniki, amakosa mu mikorere n'izindi mpamvu z'icyitegererezo.

UBUBIKO N'UBUTABE
1. Iyi mashini ishobora kumara amezi 18 ikoreshwa kuva ikozwe. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. NTUBIKORESHE. Ntukabikoreshe nyuma y'itariki ntarengwa.
2. Ntugafungure agafuka gafunze kugeza igihe uzaba witeguye gukora ikizamini, kandi ikizamini gikoreshwa rimwe gusa ni byiza ko gikoreshwa mu gihe gikenewe (ubushyuhe 2-35℃, ubushuhe 40-90%) mu minota 60 vuba bishoboka.
3. Icyitegererezo cy'umuti uvangwa n'amazi gikoreshwa ako kanya nyuma yo gufungurwa.

IMIBURO N'IBYITONDERWA
.Isanduku igomba kuba ifunze kandi irinzwe ubushuhe.
Ingero zose z’ibyavuye mu bushakashatsi zigomba kwemezwa n’ubundi buryo.
Ibipimo byose bigomba gufatwa nk'ibintu bishobora kwanduza.
NTUKORESHE reagent yarengeje igihe.
NTUGAHINDURE ibyuma bitanga ubuziranenge hagati y'ibikoresho bifite ubwoko butandukanye..
NTUGAKORESHE gukoresha amakarita yo gupima n'ibindi bikoresho byo gukoresha mu gihe cyo kuyakoresha.
.Gukoresha nabi, icyitegererezo gikabije cyangwa gito bishobora gutuma ibisubizo bihinduka.

LKWIGANA
Nkuko bigenda ku isuzuma ryose rikoresha uturemangingo tw’imbeba, birashoboka ko uturemangingo tw’imbeba (HAMA) twangiritse mu gipimo. Ingero z’abarwayi bahawe imiti y’uturemangingo tw’imbeba kugira ngo bapimwe cyangwa bavurwe zishobora kuba zirimo HAMA. Ingero nk’izo zishobora gutera ibisubizo bitari byo by’ukuri cyangwa bitari ukuri.
Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabo, amateka y'ubuzima bwabo, ubundi buryo bwo gusuzuma muri laboratwari, uko ubuvuzi bwaba buhagaze, ibyorezo by'indwara n'andi makuru.
Iyi reagent ikoreshwa gusa mu gupima amaraso mu maraso no mu maraso. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.

IBIRANGA IMIKORERE

RIBIREBANA
1.Hansen JH, et al.HAMA Interference hamwe n'ibizamini by'imyuka bishingiye ku murine monoclonal antibody [J].J bya Clin Immunoassay, 1993,16:294-299.
2.Levinson SS. Imiterere y'uturemangingo tw'umubiri duto duto n'uruhare mu gutera ikibazo cy'ubudahangarwa bw'umubiri [J].J ya Clin Immunoassay, 1992,15:108-114.

Urufunguzo rw'ibimenyetso byakoreshejwe:

	Igikoresho cyo kwa muganga gisuzuma indwara muri Vitro
	Uruganda
	Bika ahantu hari ubushyuhe buri hagati ya 2 na 30°C
	Itariki izarangiriraho
	Ntukongere gukoresha
	UBWITONDE
	Reba amabwiriza yo gukoresha

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