Ikhithi yokuxilonga ye-c-peptide
Ulwazi lwemveliso
| Inombolo yoMzekelo | I-CP | Ukupakisha | Iimvavanyo ezingama-25/ikhithi, iikhithi ezingama-30/i-CTN |
| Igama | Ikhithi yokuxilonga ye-C-peptide | Ukuhlelwa kwezixhobo | Iklasi yesiBini |
| Iimbonakalo | Uvakalelo oluphezulu, Ukusebenza lula | Isatifikethi | I-CE/ ISO13485 |
| Ukuchaneka | > 99% | Beka ubomi kwishelufa | Iminyaka emibini |
| Indlela yokusebenza | Uvavanyo lwe-Fluorescence Immunochromatographic | Inkonzo ye-OEM/ODM | Iyafumaneka |
Injongo Yokusebenzisa
Le khithi yenzelwe ukufumanisa ubungakanani be-in vitro kumxholo we-C-peptide kwi-serum/plasma/isampuli yegazi elipheleleyo lomntu kwaye yenzelwe ukufumanisa isifo seswekile kunye nomsebenzi weeseli ze-pancreatic β-cells. Le khithi inika kuphela iziphumo zovavanyo lwe-C-peptide, kwaye iziphumo ezifunyenweyo ziya kuhlaziywa kunye nolunye ulwazi lweklinikhi.
Isishwankathelo
I-C-Peptide (i-C-Peptide) yi-peptide edibanisayo eyenziwe ngama-amino acid angama-31 enobunzima bemolekyuli obumalunga nama-3021 eeDalton. Ii-β-cells zepancreas zenza i-proinsulin, eyi-protein chain ende kakhulu. I-Proinsulin yahlulwe yangamaqhekeza amathathu phantsi kwesenzo see-enzymes, kwaye amacandelo angaphambili nangasemva adityaniswa kwakhona ukuze abe yi-insulin, eyenziwe yi-A kunye ne-B chain, ngelixa icandelo eliphakathi lizimele kwaye laziwa ngokuba yi-C-peptide. I-insulin kunye ne-C-peptide zikhutshwa kumanqanaba alinganayo, kwaye emva kokungena egazini, uninzi lwe-insulin alusebenzi sisibindi, ngelixa i-C-peptide ingathathwa rhoqo sisibindi, kwaye ukuchithwa kwe-C-peptide kuhamba kancinci kune-insulin, ngoko ke uxinzelelo lwe-C-peptide egazini luphezulu kunolwe-insulin, luhlala lungaphezulu kwezihlandlo ezi-5, ngoko ke i-C-peptide ibonisa ngokuchanekileyo umsebenzi wee-β-cells ze-pancreatic islet. Ukulinganiswa kwenqanaba le-C-peptide kungasetyenziselwa ukwahlulahlula isifo seswekile kunye nokuqonda umsebenzi weeseli ze-pancreatic β-cells kwizigulane ezinesifo seswekile. Ukulinganiswa kwenqanaba le-C-peptide kungasetyenziselwa ukwahlulahlula isifo seswekile kunye nokuqonda umsebenzi weeseli ze-pancreatic β-cells kwizigulane ezinesifo seswekile. Okwangoku, iindlela zokulinganisa i-C-peptide ezisetyenziswa kakhulu kwiikliniki zonyango ziquka i-radioimmunoassay, i-enzyme immunoassay, i-electrochemiluminescence, i-chemiluminescence.
Uphawu:
• Uvakalelo oluphezulu
• ukufundwa kweziphumo kwimizuzu eli-15
• Ukusebenza okulula
• Ixabiso elithe ngqo lefektri
• kufuneka umatshini wokufunda iziphumo
Inkqubo yovavanyo
| 1 | I-1: Ukusetyenziswa kwe-analyzer ye-immune ephathekayo |
| 2 | Vula iphakheji ye-reagent yebhegi yefoyile ye-aluminiyam uze ukhuphe isixhobo sovavanyo. |
| 3 | Faka isixhobo sovavanyo ngokuthe tye kwindawo yokuhlaziya umzimba. |
| 4 | Kwiphepha lasekhaya le-interface yokusebenza ye-immune analyzer, cofa u-“Standard” ukuze ufake i-interface yovavanyo. |
| 5 | Cofa u-“QC Scan” ukuze uskene ikhowudi ye-QR kwicala langaphakathi lekhithi; iiparameter ezinxulumene nekhithi yokufaka kwisixhobo kwaye ukhethe uhlobo lwesampulu. Qaphela: Inani ngalinye lebhetshi yekhithi kufuneka liskene kube kanye. Ukuba inombolo yebhetshi iskene, ngoko tsiba eli nyathelo. |
| 6 | Jonga ukuhambelana kwe "Igama leMveliso", "Inombolo yeBatch" njl. njl. kwi-interface yovavanyo kunye nolwazi olukwileyibhile yekhithi. |
| 7 | Qalisa ukongeza isampuli xa ulwazi luhambelana:Inyathelo 1: thatha isampuli yegazi elipheleleyo eliyi-80μL kancinci kancinci, uze ungazijongi iibhamuza zegazi eliphantsi; Inyathelo lesi-2: isampulu yepipette ukuya kwisampulu ye-diluent, uze udibanise kakuhle isampulu nesampulu ye-diluent; Inyathelo lesi-3: i-pipette engama-80µL ixutywe kakuhle kwisixhobo sovavanyo, kwaye qaphela ukuba akukho maqamza e-pipette ngexesha lokuthatha isampuli |
| 8 | Emva kokongeza isampuli epheleleyo, cofa u-“Timing” kwaye ixesha eliseleyo lovavanyo liya kuboniswa ngokuzenzekelayo kwi-interface. |
| 9 | I-immune analyzer izakugqiba uvavanyo kunye nohlalutyo ngokuzenzekelayo xa ixesha lovavanyo lifikile. |
| 10 | Emva kokuba uvavanyo lwe-immune analyzer lugqityiwe, iziphumo zovavanyo ziya kuboniswa kwi-interface yovavanyo okanye zinokujongwa "kwiMbali" kwiphepha lasekhaya le-interface yokusebenza. |
