UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-CRP antibody kwindawo yovavanyo kunye ne-anti-rabbit IgG antibody yebhokhwe kwindawo yokulawula. I-Lable pad igqunywe nge-fluorescence ebhalwe nge-anti-CRP antibody kunye ne-rabbit IgG kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-antigen ye-CRP kwisampuli idibana ne-anti-CRP antibody ebhalwe nge-fluorescence, kwaye yenze umxube womzimba. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlule kwindawo yovavanyo, idibene ne-anti-CRP coating antibody, yenza i-complex entsha. Inqanaba le-CRP lihambelana kakuhle nesignali ye-fluorescence, kwaye uxinzelelo lwe-CRP kwisampuli lunokufunyanwa ngovavanyo lwe-fluorescence immunoassay.

Inkqubo yoVavanyo

Nceda ufunde incwadi yemiyalelo yokusebenza kwesixhobo kunye nesihlomelo sephakheji ngaphambi kokuvavanya.

1. Beka ecaleni zonke ii-reagents kunye neesampuli kubushushu begumbi.
2. Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokugqitha le-akhawunti ngokwendlela yokusebenza kwesixhobo, uze ufake i-interface yokubona.
3. Skena ikhowudi yokuchonga ukuze uqinisekise into yovavanyo.
4. Khupha ikhadi lovavanyo kwingxowa yefoyile.
5. Faka ikhadi lovavanyo kwindawo yokubeka ikhadi, skena ikhowudi ye-QR, uze uchonge into yovavanyo
6. Yongeza isampuli ye-serum/plasma eyi-5μL (okanye i-10μL yegazi elipheleleyo) kwisampuli yokunyibilikisa, uze udibanise kakuhle.
7. Yongeza isisombululo sesampulu se-80μL kwisampulu yomthombo wekhadi.
8. Cofa iqhosha elithi "uvavanyo oluqhelekileyo", emva kwemizuzu emi-3, isixhobo siza kuzibona ngokuzenzekelayo ikhadi lovavanyo, sifunde iziphumo kwisikrini sokubonisa isixhobo, size sirekhode/siprinte iziphumo zovavanyo.
9. Jonga imiyalelo ye-Portable Immune Analyzer (WIZ-A101).