UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-anti HbA1c antibody kwindawo yovavanyo kunye ne-anti Hb antibody kwindawo yokulawula. I-lable pad igqunywe nge-fluorescence ebhalwe nge-anti Hb antibody kunye ne-anti HbA1c antibody kwangaphambili. Xa kuvavanywa isampuli, i-antigen ye-HbA1c kwisampuli idibana ne-anti HbA1c antibody ebhalwe nge-fluorescence, i-antigen ye-Hb kwisampuli idibana ne-anti Hb antibody ebhalwe nge-fluorescence kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlula kwindawo yovavanyo, idibene ne-anti HbA1c coating antibody, yenza i-complex entsha. Xa i-complex idlula kwindawo yokulawula, idibene ne-anti Hb coating antibody, yenza i-complex entsha. Inqanaba le-HbA1c lihambelana kakuhle nesignali ye-fluorescence, kwaye uxinzelelo lwe-HbA1c kunye ne-Hb kwisampuli lunokufunyanwa ngovavanyo lwe-fluorescence immunoassay.

Inkqubo yoVavanyo:

Nceda ufunde incwadi yemiyalelo yokusebenza kwesixhobo kunye nesihlomelo sephakheji ngaphambi kokuvavanya.

1. Beka ecaleni zonke ii-reagents kunye neesampuli kubushushu begumbi.
2. Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokugqitha le-akhawunti ngokwendlela yokusebenza kwesixhobo, uze ufake i-interface yokubona.
3. Skena ikhowudi yokuchonga ukuze uqinisekise into yovavanyo.
4. Khupha ikhadi lovavanyo kwingxowa yefoyile.
5. Faka ikhadi lovavanyo kwindawo yokubeka ikhadi, skena ikhowudi ye-QR, uze uchonge into yovavanyo
6. Yongeza i-10μL yegazi elipheleleyo kwisampuli ye-diluent, shukumisa umzuzu o-1 uze udibanise kakuhle.
7. Yongeza isisombululo sesampulu se-80μL kwisampulu yomthombo wekhadi.
8. Cofa iqhosha elithi "uvavanyo oluqhelekileyo", emva kwemizuzu eli-15, isixhobo siza kuzibona ngokuzenzekelayo ikhadi lovavanyo, sifunde iziphumo kwisikrini sokubonisa isixhobo, size sirekhode/siprinte iziphumo zovavanyo.
9. Jonga imiyalelo ye-Portable Immune Analyzer (WIZ-A101).