ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-FOB antibody endaweni yokuhlola kanye ne-antibody yembuzi ye-anti rabbit IgG endaweni yokulawula. I-lable pad imbozwe nge-fluorescence ebhalwe nge-antibody ye-FOB kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula enhle, i-antigen ye-FOB kusampula ihlangana ne-antibody ye-antiFOB ebhalwe nge-fluorescence, bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza kwe-complex kuya ohlangothini lwephepha elimuncayo. Lapho i-complex idlula esifundeni sokuhlola, ihlanganiswe ne-antibody ye-antiFOB coating, yakha i-complex entsha. Uma i-negative, isampula ayinayo i-hemoglobin yomuntu noma okuqukethwe kuphansi, ama-immune complex anele awakwazi ukwakheka. Ngeke kube khona umugqa obomvu endaweni yokuthola (T). Umugqa obomvu ubonakala endaweni yokulawula ikhwalithi (C) ukuze kuhlulelwe ukuthi kukhona amasampula anele nokuthi inqubo ye-chromatography ijwayelekile. Isetshenziswa futhi njengendinganiso yokulawula yangaphakathi yama-reagents.

Inqubo Yokuhlola:

Sicela ufunde isethulo sephakheji ngaphambi kokuhlola.

1. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni elilinganayo bese ulimaka.

2. Susa isivalo epayipini lesampula bese ulahla amaconsi amabili okuqala esampula exutshwe, engeza amaconsi amathathu (cishe ama-uL ayi-100) isampula exutshwe nebhamuza eqonde phezulu futhi kancane kancane emthonjeni wesampula wekhadi nge-dispette enikeziwe. Bese uqala i-timer.

3. Umphumela kufanele ufundwe zingakapheli imizuzu eyi-10-15, futhi awusebenzi ngemva kwemizuzu eyi-15.