ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-FOB antibody endaweni yokuhlola kanye ne-anti-rabbit IgG ye-antibody yembuzi endaweni yokulawula.Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi i-anti FOB antibody kanye ne-rabbit IgG kusengaphambili.Lapho kuhlolwa isampula elihle, i-antigen ye-FOB kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti FOB antibody, bese yenza ingxube yamasosha omzimba.Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo.Lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-FOB yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha.Uma inegethivu, isampula ayinayo i-hemoglobin yomuntu noma okuqukethwe kuphansi, ama-immune complexes anele awakwazi ukwakhiwa.Ngeke kube khona umugqa obomvu endaweni yokuthola (T).Umugqa obomvu uyindinganiso evela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini.Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo Yokuhlola:

Sicela ufunde okufakiwe kwephakheji ngaphambi kokuhlola.

1.Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.

2.Khipha ikepisi kushubhu yesampula bese ulahla amathonsi amabili okuqala esampula ehlanjululiwe, engeza amaconsi ama-3 (cishe u-100uL) alikho ibhamuza elihlanjululwe isampula liqonde phezulu futhi kancane ungene emthonjeni wesampula wekhadi eline-dispette enikeziwe.Bese uqala isibali sikhathi.

3.Umphumela kufanele ufundwe phakathi kwemizuzu eyi-10-15, futhi awuvumelekile ngemuva kwemizuzu eyi-15.