ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe ngamasosha omzimba we-Hp-Ab endaweni yokuhlola kanye ne-anti-rabbit IgG yamasosha omzimba endaweni yokulawula.Iphedi elilebula limbozwe nge-fluorescence ebhalwe ukuthi i-anti Hp-Ag kanye ne-rabbit IgG kusengaphambili.Lapho kuhlolwa isampula elihle, i-Hp-Ab kusampula ihlangana ne-fluorescence enelebuli ye-anti Hp-Ag, bese yenza ingxube yokuzivikela komzimba.Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo.Lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-Hp-Ag yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha.Uma inegethivu, awekho ama-antibody e-HP kusampula, ukuze amasosha omzimba angakwazi ukwakheka, ngeke kube khona umugqa obomvu endaweni yokuthola (T).Umugqa obomvu uyindinganiso evela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini.Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo Yokuhlola:

Sicela ufunde okufakiwe kwephakheji ngaphambi kokuhlola.

1. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.

2. Engeza amaconsi angu-2 e-serum noma isampula ye-plasma (noma amaconsi angu-3 esampula legazi eliphelele/umunwe) ukuze usampula kahle ikhadi nge-dispette enikeziwe, bese wengeza iconsi elingu-1 lesampula le-diluent, qala isikhathi.

3. Linda okungenani imizuzu eyi-10-15 futhi ufunde umphumela ngemizuzu eyi-10-15.Umphumela awuvumelekile ngemva kwemizuzu engu-15.