UMGAQO

Inwebu yesixhobo sovavanyo igqunywe nge-antibody ye-Hp-Ab kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo.Iphedi elayibhile iqatywe nge-fluorescence ebhalwe i-anti Hp-Ag kunye nomvundla we-IgG kwangaphambili.Xa uvavanya isampulu eqinisekileyo, i-Hp-Ab kwisampulu idibanisa ne-fluorescence ebhalwe anti Hp-Ag, kwaye yenza umxube wokugonyeka.Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elithathayo.Xa i-complex iphumelele ummandla wovavanyo, idityaniswe ne-anti-Hp-Ag yokugquma i-antibody, yenza into entsha.Ukuba i-negative, ayikho i-antibody ye-HP kwisampuli, ukwenzela ukuba i-immune complexes ayinakwenziwa, ayiyi kuba nomgca obomvu kwindawo yokufumanisa (T).Umgca obomvu ngumgangatho ovela kwindawo yokulawula umgangatho (C) ukugweba ukuba kukho iisampuli ezaneleyo kunye nokuba inkqubo yechromatography iyinto eqhelekileyo.Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi kwii-reagents.

Inkqubo yoVavanyo:

Nceda ufunde iphakheji efakwe phambi kovavanyo.

1. Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.

2. Yongeza amathontsi ama-2 e-serum okanye isampulu yeplasma (okanye amathontsi ama-3 egazi elipheleleyo/isampula yegazi lencam yomnwe) ukwenza isampulu yekhadi kunye ne-dispette enikiweyo, emva koko wongeze i-1 ithontsi ye-diluent yesampulu, qalisa ixesha.

3. Lindela ubuncinci be-10-15 imizuzu kwaye ufunde umphumo kwi-10-15 imizuzu.Isiphumo asisebenzi emva kwemizuzu eyi-15.