UMGAQO

Inwebu yesixhobo sovavanyo igqunywe nge-antigen ye-Rotavirus yeQela A kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yolawulo.Iphedi elayibhile iqatywe nge-fluorescence ebhalwe anti Rotavirus Group A kunye nomvundla IgG kwangaphambili.Xa uvavanyo lwesampulu eqinisekileyo, i-RV kwisampulu idibanisa ne-fluorescence ebhalwe i-anti Rotavirus Group A, kwaye yenze umxube wokuzivikela komzimba.Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elithathayo.Xa i-complex iphumelele ummandla wovavanyo, idityaniswe ne-anti-Rotavirus yeQela A lokugquma i-antibody, yenza into entsha.Ukuba i-negative, akukho i-antigen ye-Rotavirus yeQela kwisampuli, ukwenzela ukuba i-immune complexes ayinakwenziwa, ayiyi kuba nomgca obomvu kwindawo yokufumanisa (T).Nokuba yeyiphi na i-rotavirus yeqela elikhoyo kumzekelo, i-mouse ebhalwe nge-latex i-IgG ifakwe kwi-chromatographed ukuya kwindawo yokulawula umgangatho (C) kwaye ibanjwe yi-anti-mouse IgG antibody.Umgca obomvu uya kubonakala kwindawo yokulawula umgangatho (C).Umgca obomvu ngumgangatho ovela kwindawo yokulawula umgangatho (C) ukugweba ukuba kukho iisampuli ezaneleyo kunye nokuba inkqubo yechromatography iyinto eqhelekileyo.Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi kwii-reagents.

Inkqubo yoVavanyo:

1.Izigulane ezineempawu kufuneka ziqokelelwe.Ngokutsho kweengxelo, ukukhutshwa okuphezulu kwe-rotavirus kwindle yezigulane ezine-gastroenteritis kwenzeka emva kweentsuku ezi-3-5 emva kokuqala kwesi sifo kunye neentsuku ezi-3-13 emva kokuqala kweempawu.Ukuba isampuli iqokelelwe ixesha elide emva kohudo, inani le-antigens lingenako ngokwaneleyo ukuba lwenzeke ukuphendula okulungileyo.

2.Iisampulu kufuneka ziqokelelwe kwisikhongozeli esicocekileyo, esomileyo, esingenamanzi esingenazo izinto zokucoca kunye nezithinteli.

3.Kwizigulane ezingarhudoyo, iisampulu zelindle eziqokelelweyo kufuneka zingabi ngaphantsi kwe-1-2 grams.Kwizigulane ezinesifo sorhudo, ukuba ilindle limanzi, nceda uqokelele ubuncinane i-1-2 ml yolwelo lwelindle.Ukuba ilindle linegazi elininzi kunye namafinyila, nceda uqokelele isampuli kwakhona.

4.Kucetyiswa ukuvavanya iisampuli ngokukhawuleza emva kokuqokelela, ngaphandle koko kufuneka zithunyelwe kwibhubhoratri kwiiyure ezingama-6 kwaye zigcinwe kwi-2-8 ° C.Ukuba iisampulu azivavanywanga kwiiyure ezingama-72, kufuneka zigcinwe kwiqondo lobushushu elingaphantsi kwe-15°C.

5.Sebenzisa ilindle elitsha kuvavanyo, kunye neisampulu zelindle elixutywe namanzi adiyiweyo okanye adiyiweyo