ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-antigen ye-Rotavirus Group A endaweni yokuhlola kanye ne-anti-rabbit IgG yembuzi endaweni yokulawula.Iphedi elilebulayo limbozwe nge-fluorescence ebhalwe ukuthi anti Rotavirus Group A kanye ne-rabbit IgG kusengaphambili.Lapho kuhlolwa isampula elihle, i-RV esesampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti Rotavirus Group A, bese yenza ingxube yokuzivikela komzimba.Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo.Lapho inkimbinkimbi idlula isifunda sokuhlola, yahlanganiswa ne-anti-Rotavirus Group A yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha.Uma iyinegethivu, ayikho i-antigen ye-Rotavirus Group A kusampula, ukuze izakhi zokuzivikela zomzimba zingakwazi ukwakheka, ngeke kube khona umugqa obomvu endaweni yokuthola (T).Kungakhathaliseki ukuthi i-rotavirus yeqembu A ikhona yini esibonelweni, igundane elinelebula le-latex i-IgG i-chromatographed ukuya endaweni yokulawula ikhwalithi (C) futhi ibanjwe i-anti-mouse IgG antibody.Kuzovela umugqa obomvu endaweni yokulawula ikhwalithi (C).Umugqa obomvu uyindinganiso evela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini.Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo Yokuhlola:

1.Iziguli ezinezimpawu kufanele ziqoqwe.Ngokwemibiko, ukuphuma okuphezulu kwe-rotavirus endle yeziguli ezine-gastroenteritis kwenzeka ezinsukwini ezingama-3-5 ngemuva kokuqala kwalesi sifo kanye nezinsuku ezingama-3-13 ngemuva kokuqala kwezimpawu.Uma isampula iqoqwa isikhathi eside ngemva kohudo, inani lama-antigen lingase linganele ukwenzeka ukusabela okuhle.

2.Amasampula kufanele aqoqwe esitsheni esihlanzekile, esomile, esingangeni manzi esingenazo izinto zokuhlanza kanye nezivimbelakuvunda.

3. Ezigulini ezingezona isifo sohudo, amasampula endle eqoqiwe akufanele abe ngaphansi kuka-1-2 amagremu.Ezigulini ezinesifo sohudo, uma indle iwuketshezi, sicela uqoqe okungenani u-1-2 ml woketshezi lwendle.Uma indle iqukethe igazi eliningi namafinyila, sicela uqoqe isampula futhi.

4.Kunconywa ukuhlola amasampula ngokushesha ngemva kokuqoqwa, ngaphandle kwalokho kufanele athunyelwe elabhorethri kungakapheli amahora angu-6 futhi agcinwe ku-2-8 ° C.Uma amasampula engazange ahlolwe phakathi namahora angu-72, kufanele agcinwe ezingeni lokushisa elingaphansi kuka -15°C.

5.Sebenzisa indle emisha ukuhlola, kanye namasampula endle axutshwe namanzi ahlanzekile noma acwecwe