ISIMISO

I-membrane yedivayisi yokuhlola ihlanganiswe ne-Tf antibody endaweni yokuhlola kanye ne-anti-rabbit IgG ye-IgG yembuzi endaweni yokulawula.Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi i-anti Tf antibody kanye ne-rabbit IgG kusengaphambili.Lapho kuhlolwa isampula elihle, i-antigen ye-Tf kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti Tf antibody, bese yenza inhlanganisela yokuzivikela komzimba.Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo.Lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti Tf enamathelayo yamasosha omzimba, yakha inkimbinkimbi entsha.

Uma inegethivu, isampula ayinayo i-transferrin noma okuqukethwe kuphansi, ukuze i-immune complex ingakwazi ukwakheka.Ngeke kube khona umugqa obomvu endaweni yokuthola (T).Kungakhathaliseki ukuthi i-antibody ye-Tf ikhona esifanekisweni noma cha, igundane elisele elibhalwe ngegolide eline-colloidal anti-human IgM monoclonal antibody kanye ne-anti-mouse IgG antibody ehlanganiswe endaweni yokulawula ikhwalithi (C).Khona-ke ama-agglutinates athuthukisa umbala endaweni yokulawula ikhwalithi, futhi umugqa obomvu uzovela ku-(C).Umugqa obomvu uyindinganiso evela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini.Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo Yokuhlola

Sicela ufunde okufakiwe kwephakheji ngaphambi kokuhlola.

1.Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.

2.Khipha ikepisi kushubhu yesampula bese ulahla amathonsi amabili okuqala esampula ehlanjululiwe, engeza amaconsi ama-3 (cishe u-100uL) alikho ibhamuza elihlanjululwe isampula liqonde phezulu futhi kancane ungene emthonjeni wesampula wekhadi eline-dispette enikeziwe.Bese uqala isibali sikhathi.