PRINCIPLE

The membrane of the test device is coated with Tf antibody on the test region and goat anti rabbit IgG antibody on the control region. Lable pad are coated by fluorescence labeled anti Tf antibody and rabbit IgG in advance. When testing positive sample, the Tf antigen in sample combine with fluorescence labeled anti Tf antibody, and form immune mixture. Under the action of the immunochromatography, the complex flow in the direction of absorbent paper. When complex passed the test region, it combined with anti Tf coating antibody, forms new complex.

If it is negative, the sample does not contain transferrin or the content is low, so that the immune complex cannot be formed. There will be no red line in the detection area (T). No matter whether Tf antibody exists in the specimen or not, the remaining colloidal gold-labeled mouse anti-human IgM monoclonal antibody and the goat anti-mouse IgG antibody coated in the quality control area (C) line. Then the agglutinates develop color in the quality control area, and the red line will appear in (C). The red line is the standard appears in the quality control area (C) for judging whether there are enough samples and whether the chromatography process is normal. It is also used as an internal control standard for reagents.

Test Procedure

Please read the package insert before testing.

1.Take out the test card from the foil bag, put it on the level table and mark it.

2.Remove the cap from the sample tube and discard the first two drops diluted sample, add 3 drops (about 100uL) no bubble diluted sample vertically and slowly into sample well of the card with provided dispette. Then start the timer.