ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-anti HbA1c antibody esifundeni sokuhlola kanye ne-anti Hb antibody esifundeni sokulawula. I-lable pad imbozwe nge-anti Hb antibody ebhalwe nge-fluorescence kanye ne-anti HbA1c antibody kusengaphambili. Lapho kuhlolwa isampula, i-antigen ye-HbA1c kusampula ihlangana ne-anti HbA1c antibody ebhalwe nge-fluorescence, i-antigen ye-Hb kusampula ihlangana ne-anti Hb antibody ebhalwe nge-fluorescence bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ohlangothini lwephepha elimuncayo, lapho i-complex idlula esifundeni sokuhlola, ihlangene ne-anti HbA1c coating antibody, yakha i-complex entsha. Lapho i-complex idlula esifundeni sokulawula, ihlangene ne-anti Hb coating antibody, yakha i-complex entsha. Izinga le-HbA1c lihlobene kahle nesignali ye-fluorescence, futhi ukuhlushwa kwe-HbA1c kanye ne-Hb kusampula kungatholakala nge-fluorescence immunoassay assay.

Inqubo Yokuhlola:

Sicela ufunde incwadi yemiyalelo yokusebenza kwethuluzi kanye nephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagent namasampula endaweni yokushisa yegumbi.
2. Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3. Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5. Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa
6. Faka i-10μL yegazi eliphelele ku-diluent yesampula, shukumisa umzuzu owodwa bese uxuba kahle.
7. Engeza isisombululo sesampula esingu-80μL emthonjeni wesampula wekhadi.
8. Chofoza inkinobho ethi "ukuhlolwa okujwayelekile", ngemva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
9. Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).