ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-microalbumin antigen endaweni yokuhlola kanye ne-antibody yembuzi ye-anti rabbit IgG endaweni yokulawula. I-lable pad imbozwe nge-microalbumin ebhalwe nge-fluorescence kanye ne-rabbit IgG kusengaphambili. Uma kungekho-albumin emchameni, i-antibody ye-monoclonal ene-colloidal gold-labeled anti-Alb ebhalwe nge-colloidal gold ephepheni le-colloidal gold izogijima e-membrane nomchamo uye emgqeni wokuthola, bese ihlangana ne-antigen ene-Alb enomugqa obonakalayo. Futhi umbala womugqa umnyama kunombala womugqa endaweni yokulawula (C), lokhu kuwumphumela omubi. Uma umchamo uqukethe i-albumin, uzoncintisana ne-antigen ene-Alb eboshwe e-membrane ukuze ibophe ezindaweni ze-antibody ezilinganiselwe ku-antibody ene-colloidal gold-labeled anti-Alb. Njengoba inani le-albumin emchameni landa, ukuhlolwa

Umbala womugqa uzoba lula futhi ukhanye. Okuqukethwe kwe-albumin emchameni kungatholakala ngokwesilinganiso ngokuqhathanisa indawo yokuthola (T) nendawo yokulawula (C). Indawo yokulawula ikhwalithi (C) nendawo yokubhekisela (R) ekhithini kuzohlala kuvela ngesikhathi sokuhlolwa, futhi akunalutho oluphathelene nokuba khona kwe-albumin yomchamo. Indawo yokulawula (C) kanye nomugqa wendawo yokubhekisela (R) kungasetshenziswa njengenkomba yokubhekisela yokulawula ikhwalithi yangaphakathi yekhithi.

Inqubo Yokuhlola:

Sicela ufunde incwadi yokusebenza kwethuluzi kanye nesembozo sephakheji ngaphambi kokuhlola. Ncibilikisa amasampula abe yizinga lokushisa legumbi ngaphambi kokusebenzisa.

1. Khipha ikhadi lokuhlola esikhwameni se-foil. Libeke liqonde phezulu bese ulimaka.

2. Thatha isampula yomchamo ngepayipi elilahlwayo, lahla amaconsi amabili okuqala esampula yomchamo. Engeza amaconsi amathathu (cishe ama-uL ayi-100) omchamo ongenamagwebu phakathi nendawo yembobo yesampula yekhadi lokuhlola ngokuqondile bese uqala isikhathi.

3. Funda umphumela ngemizuzu eyi-10-15. Akuvumelekile uma kungaphezu kwemizuzu eyi-15.